Chromatin immunoprecipitation assay of Piwi in Drosophila.
Chromatin immunoprecipitation assay of Piwi in Drosophila.
复制标题
果蝇中 Piwi 的染色质免疫沉淀测定。
DOI：
10.1007/978-1-62703-694-8_1
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Lin,Haifan
中科院分区：
文献类型：
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作者：
Yin,Hang;Lin,Haifan
高分辨率表观基因组图谱的生成对于表观遗传学、发育生物学和干细胞生物学的研究至关重要。近年来，小RNA途径已被牵连在表观遗传调控。所有小RNA途径都涉及Argonaute蛋白作为其关键的生物发生和效应成分。在这一章中，我们描述了一种染色质免疫沉淀法的全基因组定位ofDrosophilaPiwi，Argonaute蛋白家族的定义成员。这种方法应具有普遍的实用性映射其他染色质相关的因素。
The generation of high-resolution maps of the epigenome is crucial to research in epigenetics, developmental biology, and stem cell biology. In recent years, small RNA pathways have been implicated in epigenetic regulation. All small RNA pathways involve Argonaute proteins as their key biogenesis and effector components. In this chapter, we describe a chromatin immunoprecipitation method for whole-genome mapping ofDrosophilaPiwi, the defining member of the Argonaute protein family. This method should have general utility for mapping other chromatin-associated factors.