Paeonol Ameliorates Abdominal Aortic Aneurysm Progression by the NF-κB Pathway
Paeonol Ameliorates Abdominal Aortic Aneurysm Progression by the NF-κB Pathway
复制标题
丹皮酚通过 NF-κB 途径改善腹主动脉瘤进展。
DOI：
10.1016/j.avsg.2021.06.003
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发表时间：
2021-12-18
影响因子：
1.5
通讯作者：
Li, Gang
中科院分区：
文献类型：
--
作者：
Chen, Shuxiao;Luo, Kun;Li, Gang
目的：腹主动脉瘤（AAA）是一种以局部进行性扩张为特征的慢性炎症性疾病。目前，丹皮酚已被证明具有抗炎和保护心血管的特性。本研究旨在探讨丹皮酚对AAA进展的潜在影响。方法：采用猪胰弹性酶主动脉内灌注法在C57BL/6J小鼠体内建立实验性AAAs，然后灌胃丹皮酚（20 mg/kg/d），持续14 d。采用超声显像、组织病理学、western blot等方法观察丹皮酚对实验性AAA的影响。结果：丹皮酚抑制了动脉瘤直径的增大，减轻了弹性纤维和血管平滑肌细胞的损耗。此外，给药后CD68+巨噬细胞和CD8+淋巴细胞的浸润明显减少，并伴有壁新生血管生成。Western blot结果显示，丹皮酚抑制基质金属蛋白酶（MMP）的表达和NF-kappa B通路的激活。结论：丹皮酚可能通过抑制nf - κ B通路抑制实验性AAA进展，提示丹皮酚是治疗AAA的潜在药物。
Objective: Abdominal aortic aneurysm (AAA) is a chronic inflammatory disease characterized by localized progressive dilatation. Currently, paeonol has been shown to possess anti-inflammatory and protective cardiovascular properties. Our study aimed to investigate the potential influences of paeonol on AAA progression.Methods: Experimental AAAs were created in C57BL/6J mice by intra-aortic infusion of porcine pancreatic elastase, and then intragastrically administered paeonol (20 mg/kg/day) for 14 days. The effects of paeonol on experimental AAA were measured by ultrasound imaging, histopathology, and western blot analyses.Results: Paeonol treatment limited the enlargement of the aneurysmal diameter and alleviated the depletion of elastic fibers and vascular smooth muscle cells (VSMCs). Furthermore, the infiltration of CD68+ macrophages and CD8+ lymphocytes was obviously attenuated after paeonol administration, along with mural neoangiogenesis. Western blot results showed that paeonol inhibited the expression of matrix metalloproteinase (MMP) and the NF-kappa B pathway activation.Conclusions: Paeonol might prevent experimental AAA progression by inhibiting the NF-kappa B pathway, which suggests that it is a potential drug for AAA.