A simple microfluidic device for live-imaging of the vertical section of epithelial cells
A simple microfluidic device for live-imaging of the vertical section of epithelial cells
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DOI：
10.1039/c9an02165e
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发表时间：
2020-01-21
期刊：
影响因子：
4.2
通讯作者：
Suzuki，Hiroaki
中科院分区：
文献类型：
--
作者：
Araki，Seigo;Nakano，Masayoshi;Suzuki，Hiroaki
我们研究了具有垂直侧壁的沟槽的简单微流体装置用于贴壁细胞活细胞荧光成像的能力。培养形成二维（2D）片的上皮细胞系，使其粘附在垂直侧壁上，以便可以使用普通倒置型激光扫描显微镜直接对其垂直切片进行成像。对该装置的材料和结构进行了表征。我们表明，细胞内细胞器（例如微管和线粒体）以及细胞间装置（例如密蛋白和闭合小带）的详细分布可以通过单次扫描以高时空分辨率成像。
We investigated the capability of simple microfluidic devices with trenches having vertical sidewalls for live-cell fluorescence imaging of adherent cells. An epithelial cell line that forms a two-dimensional (2D) sheet was cultured to adhere to the vertical sidewall so that its vertical section can be imaged directly using ordinal inverted-type laser-scanning microscopy. The material and the structure of the device were characterized. We show that the detailed distribution of intracellular organelles, such as microtubules and mitochondria, and of intercellular apparatus, such as claudin and zonula occludens, can be imaged with high spatio-temporal resolution with a single scan.