Gold nanoparticles as a versatile platform for optimizing physicochemical parameters for targeted drug delivery
Gold nanoparticles as a versatile platform for optimizing physicochemical parameters for targeted drug delivery
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DOI：
10.1002/mabi.200600075
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发表时间：
2006-07-14
影响因子：
4.6
通讯作者：
Pun, Suzie H.
中科院分区：
文献类型：
--
作者：
Bergen, Jamie M.;Von Recum, Horst A.;Pun, Suzie H.
用于全身给药的靶向载体的开发依赖于优化细胞靶向配体和纳米颗粒载体的物理化学特性。提出了一种基于巯基化聚合物修饰金纳米粒子的通用平台，其中设计参数可以独立和系统地变化。通过将PEG-硫醇和半乳糖-PEG-硫醇缀合至金胶体来制备不同粒径、表面电荷、表面亲水性和半乳糖配体密度的纳米颗粒制剂。该平台用于筛选证明体内肝细胞靶向递送的纳米颗粒制剂。发现纳米颗粒尺寸和半乳糖配体的存在显著影响靶向效率。因此，该平台可以很容易地应用于确定靶向药物递送系统的设计参数。
The development of targeted vehicles for systemic drug delivery relies on optimizing both the cell-targeting ligand and the physicochemical characteristics of the nanoparticle carrier. A versatile platform based on modification of gold nanoparticles with thiolated polymers is presented in which design parameters can be varied independently and systematically. Nanoparticle formulations of varying particle size, surface charge, surface hydrophilicity, and galactose ligand density were prepared by conjugation of PEG-thiol and galactose-PEG-thiol to gold colloids. This platform was applied to screen for nanoparticle formulations that demonstrate hepatocyte-targeted delivery in vivo. Nanoparticle size and the presence of galactose ligands were found to significantly impact the targeting efficiency. Thus, this platform can be readily applied to determine design parameters for targeted drug delivery systems.