Stimulation of neurotrophic factor secretion from 1321N1 human astrocytoma cells by novel diterpenoids, scabronines A and G.
Stimulation of neurotrophic factor secretion from 1321N1 human astrocytoma cells by novel diterpenoids, scabronines A and G.
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DOI：
10.1016/s0014-2999(99)00077-1
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发表时间：
1999-04
影响因子：
5
通讯作者：
Y. Obara;N. Nakahata;T. Kita;Y. Takaya;H. Kobayashi;S. Hosoi;F. Kiuchi;T. Ohta;Y. Oshima-
中科院分区：
文献类型：
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作者：
Y. Obara;N. Nakahata;T. Kita;Y. Takaya;H. Kobayashi;S. Hosoi;F. Kiuchi;T. Ohta;Y. Oshima-
神经胶质细胞释放维持神经元功能的神经营养因子。当大鼠嗜铬细胞瘤细胞（PC-12）与人星形细胞瘤细胞（1321 N1）的条件培养基一起培养时，培养液中含有新的二萜类化合物，scabronines A和G，分离自Sarcodon scabrosus，它们改变了形态，并有神经突生长。结果表明，糙伏毛碱能促进1321 N1细胞神经生长因子（NGF）mRNA的表达，并呈浓度依赖性地促进1321 N1细胞分泌NGF。然而，由条件培养基产生的增强的神经突生长受到NGF中和抗体的轻微抑制，并且响应于scabronines释放的NGF浓度不足以引起分化。这些结果表明，scabronines引起其他因子与NGF从1321 N1细胞的分泌。二萜类化合物是阐明神经营养因子合成和分泌机制的有用药物。
Glial cells release neurotrophic factors that maintain neurons functionally. When rat pheochromocytoma cells (PC-12) were cultivated with the conditioned medium of human astrocytoma cells (1321N1) incubated with the new diterpenoids, scabronines A and G, isolated from Sarcodon scabrosus, they changed their morphology and there was neurite outgrowth. The scabronines increased the expression of mRNA for nerve growth factor (NGF), and the secretion of NGF from 1321N1 cells in a concentration-dependent manner. However, the enhanced neurite outgrowth produced by the conditioned media was slightly inhibited by NGF neutralizing antibody, and the concentration of NGF released in response to the scabronines was insufficient to cause differentiation. These results suggest that scabronines cause the secretion of other factors together with NGF from 1321N1 cells. The diterpenoids are useful drugs to clarify the mechanism of synthesis and secretion of neurotrophic factors.