Cell uptake, cytoplasmic diffusion and nuclear access of a 6.5 nm diameter dendrimer.
Cell uptake, cytoplasmic diffusion and nuclear access of a 6.5 nm diameter dendrimer.
复制标题
6.5 nm 直径树枝状聚合物的细胞摄取、细胞质扩散和核进入。
DOI：
10.1016/j.ijpharm.2006.12.012
复制
发表时间：
2007
影响因子：
5.8
通讯作者：
Ruenraroengsak P
中科院分区：
文献类型：
--
作者：
Ruenraroengsak P
Macromolecular crowding and the presence of organelles in the cytosol present barriers to particle mobility, such that it is unclear how nano-carriers can deliver their active agents to the nucleus. In this work a sixth generation amino terminated polyamide polylysine dendrimer (Gly-Lys63(NH2)64) (MW 8149, diameter 6.5nm) which is fluorescent allowed the study of nuclear uptake and mobility in living lung carcinoma (SK/MES-1) and colon adenocarcinoma (Caco-2) cells. The dendrimer is found within 25–45min of incubation inside the cell nuclei. Living cells were then used to develop a method for the dynamic nuclear uptake study using confocal microscopy. The dynamic uptake of the dendrimer demonstrated here allowed the apparent cytoplasmic diffusion coefficient (D) of the dendrimer to be calculated. Values were found in the range 5.99×10−11cm2s−1(SK/MES-1 cells) to 9.82×10−11cm2s−1(Caco-2 cells). The difference must reflect variation in the intracellular architecture of the cell types.
登录
查看更多内容
乳胶纳米球的扩散和 HPMC 凝胶的有效（微观）粘度。
影响因子：
5.8
作者：
P. Ruenraroengsak;A. Florence
通讯作者：
A. Florence
用绿色荧光蛋白监测蛋白质分泌。
影响因子：
--
作者：
C. Kaether;H. Gerdes
通讯作者：
H. Gerdes
亲脂性聚酰胺树枝状聚合物的合成及理化性质
影响因子：
3.7
作者：
T. Sakthivel;I. Toth;A. Florence
通讯作者：
A. Florence
影响因子：
3.4
作者：
Swaminathan, R;Hoang, CP;Verkman, AS
通讯作者：
Verkman, AS
DOI：
10.1073/pnas.84.14.4910
发表时间：
1987-07-01
影响因子：
11.1
作者：
LUBYPHELPS, K;CASTLE, PE;LANNI, F
通讯作者：
LANNI, F