Homocysteine concentrations in follicular fluid are associated with poor oocyte and embryo qualities in polycystic ovary syndrome patients undergoing assisted reproduction
Homocysteine concentrations in follicular fluid are associated with poor oocyte and embryo qualities in polycystic ovary syndrome patients undergoing assisted reproduction
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DOI：
10.1093/humrep/dep069
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发表时间：
2009-09-01
影响因子：
6.1
通讯作者：
Satiroglu, Hakan
中科院分区：
文献类型：
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作者：
Berker, Buelent;Kaya, Cemil;Satiroglu, Hakan
多囊卵巢综合征（PCOS）患者卵母细胞和胚胎质量差，受精率低。卵泡液同型半胱氨酸（Hcy）水平与卵母细胞和胚胎质量之间也存在负相关。我们研究了辅助生殖的PCOS患者卵泡液Hcy浓度与卵母细胞和胚胎质量的关系。52例PCOS患者被纳入研究，并接受GnRH激动剂/重组FSH治疗。取卵时测定单个卵母细胞卵泡液中的Hcy、叶酸、维生素B-12、丙二醛（MDA）和雌二醇（E-2）水平。每个卵巢取样一个卵泡，分析94个卵泡。血浆激素也被测量。卵母细胞和胚胎按标准方法分级（1-3）。血浆中Hcy、E-2、维生素B-12、叶酸和MDA浓度均高于卵泡液（P < 0.001）。3级和2级卵母细胞的卵泡Hcy水平差异有统计学意义（P < 0.001）。1 ~ 2级胚的Hcy水平低于3级胚；卵泡液Hcy浓度高的患者卵泡液维生素B-12水平较低（P < 0.01）。卵泡液Hcy水平与卵泡液维生素B-12 （r = -0.44）、叶酸（r = -0.68）、受精率（r = -0.85）呈负相关，与MDA呈正相关（r = 0.51）。在辅助生殖的PCOS患者中，卵泡液中Hcy的浓度可能是受精率、卵母细胞和胚胎质量的有用标志。
A poor quality of oocytes and embryos and a low fertilization rate have been found in polycystic ovary syndrome (PCOS) patients. An inverse association between follicular fluid homocysteine (Hcy) levels and oocyte and embryo quality has also been demonstrated. We examined the relationship between follicular fluid Hcy concentrations and oocyte and embryo quality in PCOS patients undergoing assisted reproduction.Fifty-two PCOS patients were included in the study, and underwent GnRH agonist/recombinant FSH treatment. The Hcy, folate, vitamin B-12, malonyldialdehyde (MDA) and estradiol (E-2) levels were measured in follicular fluid from single oocytes at time of retrieval. One follicle per ovary was sampled and 94 were analysed. Plasma hormones were also measured. Oocytes and embryos were graded (1-3) using standard approaches.The concentrations of Hcy, E-2, vitamin B-12, folate and MDA in plasma were higher than in follicular fluid (all P < 0.001). Significant differences were observed in follicular Hcy levels between Grade 3 and Grade 2 oocytes (P < 0.001). Hcy levels were lower in Grade 1-2 embryos than that in Grade 3 embryos; follicular fluid vitamin B-12 levels were lower in patients showing high concentrations of follicular fluid Hcy (P < 0.01). The follicular fluid Hcy levels were negatively correlated with follicular fluid vitamin B-12 (r = -0.44), folate (r = -0.68) and fertilization rate (r = -0.85), and positively correlated with follicular fluid MDA (r = 0.51).Concentrations of Hcy in follicular fluid on the dOPU may be a useful marker for fertilization rate, and oocyte and embryo quality in PCOS patients undergoing assisted reproduction.