SEPARABLE REGULATORY ELEMENTS MEDIATE THE ESTABLISHMENT AND MAINTENANCE OF CELL STATES BY THE DROSOPHILA SEGMENT-POLARITY GENE GOOSEBERRY
SEPARABLE REGULATORY ELEMENTS MEDIATE THE ESTABLISHMENT AND MAINTENANCE OF CELL STATES BY THE DROSOPHILA SEGMENT-POLARITY GENE GOOSEBERRY
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DOI：
10.1002/j.1460-2075.1993.tb05786.x
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发表时间：
1993-04-01
期刊：
影响因子：
11.4
通讯作者：
NOLL, M
中科院分区：
文献类型：
--
作者：
LI, XL;GUTJAHR, T;NOLL, M
在果蝇胚胎发生过程中，沿着前后轴的沿着在每个片段内由片段极性基因的产物指定，所述片段极性基因包括wingless（wg）和gooseberry（gsb）。这些基因在每个片段中的条纹状表达最初是由胚盘晚期的成对规则基因产物建立的。这种模式是随后保持芽带延伸后，通过相互作用之间的片段极性基因本身。在这里，我们表明，gsb，PHox基因编码的配对结构域和同源结构域的维护，是由wg信号，小鼠Wnt-1蛋白的同源物控制。负责wg依赖性维持gsb表达的控制元件gsb晚期元件与成对规则转录因子初始激活gsb所需的元件gsb早期元件是可分离的。这样的监管策略的意义进行了讨论，相对于建立和维护细胞状态内的每一段由段极性基因。
During Drosophila embryogenesis, position along the anteroposterior axis is specified within each segment by the products of the segment-polarity genes which include wingless (wg) and gooseberry (gsb). The striped expression of these genes in each segment is initially established by the pair-rule gene products during late blastoderm. This pattern is subsequently maintained after germ band extension by interaction among the segment-polarity genes themselves. Here we show that the maintenance of gsb, a PHox gene encoding a paired-domain and a homeodomain, is controlled by the wg signal, the homolog of the murine Wnt-1 protein. A control element responsible for wg-dependent maintenance of gsb expression, gsb-late element, is separable from an element required for the initial activation of gsb by pair-rule transcription factors, gsb-early element. The significance of such a regulatory strategy is discussed with respect to the establishment and maintenance of cell states within each segment by segment-polarity genes.