FORMATION OF A SUPRAMOLECULAR COMPLEX IS INVOLVED IN THE RECONSTITUTION OF BASEMENT-MEMBRANE COMPONENTS
FORMATION OF A SUPRAMOLECULAR COMPLEX IS INVOLVED IN THE RECONSTITUTION OF BASEMENT-MEMBRANE COMPONENTS
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DOI：
10.1021/bi00290a014
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发表时间：
1983-01-01
期刊：
影响因子：
2.9
通讯作者：
MARTIN, GR
中科院分区：
文献类型：
--
作者：
KLEINMAN, HK;MCGARVEY, ML;MARTIN, GR
基底膜大分子，包括IV型胶原蛋白、层粘连蛋白和硫酸乙酰肝素蛋白聚糖，单独孵育时不聚集。沉淀发生在等摩尔量的层粘连蛋白和IV型胶原蛋白，但硫酸乙酰肝素蛋白多糖的变量的存在下。这种相互作用需要天然层粘连蛋白和IV型胶原蛋白。硫酸乙酰肝素蛋白聚糖增加层粘连蛋白的沉淀，特别是在存在IV型胶原的情况下。纤连蛋白不引起IV型胶原沉淀。基底膜的组分以高度特异性的方式相互作用。这种相互作用可能参与了基底膜的原位沉积。
Basement membrane macromolecules, including type-IV collagen, laminin and heparan sulfate proteoglycan, do not aggregate when incubated alone. Precipitation occurs in the presence of equimolar amounts of laminin and type-IV collagen but variable amounts of heparan sulfate proteoglycan. This interaction requires native laminin and type-IV collagen. Heparan sulfate proteoglycan increases the precipitation of laminin particularly in the presence of type-IV collagen. Fibronectin does not cause type-IV collagen to precipitate. Components of basement membrane interact in a highly specific manner. Such interactions may be involved in the deposition of basement membrane in situ.