A multifunctional basic pH indicator probe for distinguishable detection of Co2+, Cu2+ and Zn2+ with its utility in mitotracking and monitoring cytoplasmic viscosity in apoptotic cells.
A multifunctional basic pH indicator probe for distinguishable detection of Co2+, Cu2+ and Zn2+ with its utility in mitotracking and monitoring cytoplasmic viscosity in apoptotic cells.
复制标题
一种多功能碱性 pH 指示剂探针，可区分检测 Co2、Cu2 和 Zn2，并可用于线粒体追踪和监测凋亡细胞的细胞质粘度。
DOI：
10.1039/d2dt00286h
复制
发表时间：
2022
影响因子：
4
通讯作者：
Vinod P. Singh
中科院分区：
文献类型：
--
作者：
Pranjalee Yadav;Sarita Gond;Anusmita Shekher;Subash C. Gupta;U. Singh;Vinod P. Singh
Co2+、Cu2+、Zn2+等金属离子在生物和工业领域有着广泛的应用，但这些离子产生的毒性对人类构成严重威胁。然而，文献中没有关于开发用于可区分检测这些有毒离子的化学传感器的报道。为了解决这一挑战，据报道，一种多功能探针作为基本 pH 指示剂，具有比色和荧光开启响应。该探针在 DMF : 水（9 : 1 v/v，HEPES 10 mM）中选择性地区分分别为棕色、深黄色和绿黄色的 Co2+、Cu2+ 和 Zn2+ 离子。此外，还观察到特定于 Zn2+ 的荧光开启响应。使用紫外-可见光、荧光光谱和 1H NMR 滴定探索了传感机制，并通过计算结果得到了证实。 C·N 异构化和激发态分子内质子转移 (ESIPT) 的抑制以及螯合增强荧光发射 (CHEF) 导致 Zn2+ 的荧光增强。乔布图和 HRMS 谱证实了探针和金属离子之间的 1 : 1 (L : M) 化学计量。该探针能够在DMF :甘油介质中表现出优异的粘变色性。 SiHa 细胞的活细胞成像已成功用于碱性 pH 下 Zn2+ 的细胞内检测。此外，该探针在 SiHa 细胞中线粒体追踪和细胞质粘度变化方面显示出其实用性。它通过显示从癌性 SiHa 细胞到凋亡细胞的荧光强度增强，有效地用于识别细胞凋亡过程。
Metal ions such as Co2+, Cu2+ and Zn2+ have extensive applications in biological and industrial realms, but the toxicity caused by these ions poses a serious threat to mankind. However, there is no report in the literature on the development of a chemosensor for distinguishable detection of these toxic ions. Addressing this challenge, a multifunctional probe as a basic pH indicator with both colorimetric and fluorescence turn-on responses has been reported. The probe selectively discriminates Co2+, Cu2+ and Zn2+ ions with brown, dark yellow and greenish yellow colors, respectively, in DMF : water (9 : 1 v/v, HEPES 10 mM). Additionally, a fluorescence turn-on response specific to Zn2+ has also been observed. The sensing mechanism has been explored using UV-Vis, fluorescence spectroscopy and 1H NMR titration and confirmed with computational results. The inhibition of CN isomerization and excited state intramolecular proton transfer (ESIPT) along with chelation enhanced fluorescence emission (CHEF) result in fluorescence enhancement with Zn2+. Job's plot and HRMS spectra confirm a 1 : 1 (L : M) stoichiometry between the probe and metal ions. The probe is able to exhibit excellent viscochromism in DMF : glycerol medium. Live cell imaging on SiHa cells has been successfully performed for intra-cellular detection of Zn2+ at basic pH. Furthermore, the probe displays its utility in mitotracking and monitoring cytoplasmic viscosity changes in SiHa cells. It is efficiently used to recognize the apoptosis process by displaying an enhancement in fluorescence intensity from cancerous SiHa cells to apoptotic cells.