MORPHOLOGICAL OBSERVATIONS ON MUCUS-SECRETING NONGOBLET CELLS IN THE DEEP CRYPTS OF THE RAT ASCENDING COLON
MORPHOLOGICAL OBSERVATIONS ON MUCUS-SECRETING NONGOBLET CELLS IN THE DEEP CRYPTS OF THE RAT ASCENDING COLON
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DOI：
10.1002/aja.1001670109
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发表时间：
1983-01-01
影响因子：
--
通讯作者：
ALTMANN, GG
中科院分区：
文献类型：
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作者：
ALTMANN, GG
在成年雄性大鼠的升结肠中，观察到大量的分化的粘液型细胞聚集，几乎完全形成深1/2-1/3的隐窝。有丝分裂活动定位于中隐窝，柱状细胞。将粘液型细胞命名为深隐窝分泌型细胞（DCS），并对其进行了组织学研究。在光学显微镜下，顶端的细胞质膨胀与粘液空泡后，使用过碘酸希夫（PAS），阿辛蓝或马森染色后，染色不同于杯状细胞。在电镜下，发达的高尔基复合体和粗面内质网，以及大量的电子不透明的空泡，表明糖蛋白的积极生产。用秋水仙素技术和~ 3 H-胸腺嘧啶核苷放射自显影进一步证实了中隐窝有丝分裂活动。从中隐窝到隐窝基底，DCS细胞中粘液含量逐渐增加，核和核仁大小逐渐减小，表明这些细胞起源于中隐窝，并随着它们向隐窝基底的进展而成熟。细胞计数显示，构成隐窝的细胞数量在整个沿着结肠大致相同，但DCS细胞的百分比不同：升结肠为33%，横结肠为21%。在降结肠和盲肠中分别仅发现5%和8%的深隐窝粘液型细胞，但这些细胞在外观上不同于成熟的DCS细胞。在升结肠的深隐窝中发现了大量具有强烈分泌活性的非有丝分裂终细胞。这些细胞与空泡化或其他上皮细胞类型的确切关系仍有待进一步研究。
In the ascending colon of adult male rats, large accumulations of differentiated, mucous-type cells were observed which formed the deep 1/2-1/3 of the crypts almost exclusively. Mitotic activity was localized to the midcrypt, to columnar-type cells. The mucous-type cells were designated as deep crypt secretory (DCS), and their histological study was initiated. In the light microscope, the apical cytoplasm was distended with mucous vacuoles which stained differently from goblet cells after using periodic acid-Schiff (PAS), alcian blue or a Masson stain. In electronmicrographs, a well-developed Golgi complex and rough endoplasmic reticulum in addition to a large number of electron-opaque vacuoles indicated active production of a glycoprotein. The midcrypt mitotic activity was further demonstrated by using the colchicine technique and 3H-thymidine radioautography. A gradual increase of mucous content and a decrease of nuclear and nucleolar size in the DCS cells from midcrypt to crypt base indicated that these cells originate in midcrypt and mature as they progress toward the crypt base. Cell counts showed that the number of cells constituting the crypts was about the same all along the colon, but the percentage of DCS cells varied: 33% in ascending and 21% in transverse colon. Only 5% and 8% deep-crypt mucous-type cells were found in descending colon and cecum, respectively; but these cells differed in appearance from the mature DCS cells. The presence of a large number of nonmitotic end cells with intense secretory activity was ascertained for the deep crypts of the ascending colon. The exact relation of these cells to the vacuolated or other epithelial cell types remains for further study.