The Notochord Breaks Bilateral Symmetry by Controlling Cell Shapes in the Zebrafish Laterality Organ
The Notochord Breaks Bilateral Symmetry by Controlling Cell Shapes in the Zebrafish Laterality Organ
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DOI：
10.1016/j.devcel.2014.11.003
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发表时间：
2014-12-22
影响因子：
11.8
通讯作者：
Heisenberg, Carl-Philipp
中科院分区：
文献类型：
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作者：
Compagnon, Julien;Barone, Vanessa;Heisenberg, Carl-Philipp
Kupffer囊泡（KV）是斑马鱼的侧化器官，沿着胚胎的左右（LR）轴沿着形成图案。在腔衬里KV上皮细胞形状的区域差异是必不可少的LR图案功能。然而，KV细胞获得其特征形状的过程在很大程度上是未知的。在这里，我们表明，脊索诱导区域差异KV内的细胞形状触发细胞外基质（ECM）积累邻近的前背（AD）区域KV。这种局部ECM沉积限制了管腔生长期间KV AD区域中管腔衬里上皮细胞的顶端扩张。我们的研究提供了机械洞察的过程中，KV翻译全球胚胎图案到区域细胞形状差异所需的LR破膜功能。
Kupffer's vesicle (KV) is the zebrafish organ of laterality, patterning the embryo along its left-right (LR) axis. Regional differences in cell shape within the lumen-lining KV epithelium are essential for its LR patterning function. However, the processes by which KV cells acquire their characteristic shapes are largely unknown. Here, we show that the notochord induces regional differences in cell shape within KV by triggering extracellular matrix (ECM) accumulation adjacent to anterior-dorsal (AD) regions of KV. This localized ECM deposition restricts apical expansion of lumen-lining epithelial cells in AD regions of KV during lumen growth. Our study provides mechanistic insight into the processes by which KV translates global embryonic patterning into regional cell shape differences required for its LR symmetry-breaking function.