Elevated plasma F2-isoprostanes in patients on long-term hemodialysis
Elevated plasma F2-isoprostanes in patients on long-term hemodialysis
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DOI：
10.1046/j.1523-1755.2001.0590051960.x
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发表时间：
2001-05-01
影响因子：
19.6
通讯作者：
Blumberg, JB
中科院分区：
文献类型：
--
作者：
Handelman, GJ;Walter, MF;Blumberg, JB
背景接受长期血液透析（HD）的终末期肾病（ESRD）患者可能会受到由HE或肾衰竭引起的氧化应激增加。血浆F2-异前列腺素已被确立为体内氧化应激的重要指标。采用气相色谱-质谱-负化学电离（GC-MS-NCI）法测定了25例KD患者和23例肾功能正常对照者血浆酯化F2-异前列腺素。C-反应蛋白（CRP）测定同时在患者和对照组的酶联免疫吸附试验（ELISA）。α-生育酚。同时测定血清视黄醇、白蛋白和肌酐。ESRD患者的平均总酯化F2-异前列烷为1.62 +/- 0.73，对照组为0.27 +/- 0.10 ng/mL（P < 0.001），患者和对照组之间无重叠。糖尿病ESRD患者的血浆F2-异前列腺素与其他原因导致肾衰竭的患者的血浆F2-异前列腺素相似。在首次测量后8个月评价的10例ESRD患者的亚组中。血浆酯化的F2-异前列腺素不被单独的透析过程改变。血透患者血浆CRP平均值也明显高于对照组（P < 0.02）。但一些患者的CRP值与对照组相似。I-ID患者血浆总F2-异前列腺素与血浆CRP相关（I = 0.48，P = 0.015）。血浆顺式生育酚在患者和对照组之间没有差异，但患者的血浆视黄醇（3.15 +/- 1.71 μ mol/L）高于对照组（1.97 +/- 0.51 μ mol/L，P < 0.05）。这些结果与ESRD患者的氧化应激导致酯化血浆F2-异前列烷值升高的假设一致，同时部分患者的血浆CRP水平升高。酯化F2-异前列腺素清除受损可能导致肾衰竭时水平升高。血浆酯化F2-异前列腺素可能是一个有用的指标，以评估干预措施的有效性，以减少氧化应激和相关的炎症。
Background. End-stage renal disease (ESRD) patients on long term hemodialysis (HD) may be under increased oxidative stress, caused by either HE or renal failure. Plasma F2-isoprostanes have been established as an important indicator of in vivo oxidative stress.Methods. Plasma esterified F2-isoprostanes were measured in 25 KD patients and 23 controls with normal renal function, employing gas chromatography-mass spectrometry with negative chemical ionization (GC-MS-NCI). C-reactive protein (CRP) was determined concurrently in patients and controls by enzyme-linked immunosorbent assay (ELISA). alpha -Tocopherol. retinol, albumin and creatinine were also determined.Results. The average total esterified F2-isoprostanes in the ESRD patients was 1.62 +/- 0.73 vs. 0.27 +/- 0.10 ng/mL in controls (P < 0.001), with no overlap between patients and controls. Plasma F2-isoprostanes in diabetic ESRD patients were similar to F2-isoprostanes in patients with other causes for renal failure, In a subset of 10 of these ESRD patients evaluated eight months after the initial measurement. plasma-esterified F2-isoprostanes were not altered by an individual dialysis session. Average plasma CRP values were also higher in HD patients (P < 0.02). but some patients had CRP values that were similar to controls. In the I-ID patients, total plasma F2-isoprostanes and plasma CRP were correlated (I = 0.48, P = 0.015). Plasma cw-tocopherol did not differ between patients and controls, but plasma retinol was higher in patients (3.15 +/- 1.71 mu mol/L) than in controls (1.97 +/- 0.51 mu mol/L, P < 0.05).Conclusions. These results are consistent with the hypothesis that oxidative stress in ESRD patients contributes to increased values of esterified plasma F2-isoprostanes, with concurrent increases in plasma CRP levels in some patients. Impaired clearance of esterified F2-isoprostanes may contribute to the elevated levels in renal failure. Plasma esterified F2-isoprostanes may be a useful indicator to evaluate effectiveness of interventions to decrease oxidative stress and associated inflammation.