Comparative analysis of mitochondrial genomes among four species of muscid flies (Diptera: Muscidae) and its phylogenetic implications
Comparative analysis of mitochondrial genomes among four species of muscid flies (Diptera: Muscidae) and its phylogenetic implications
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四种蝇类（双翅目：蝇科）线粒体基因组的比较分析及其系统发育意义
DOI：
10.1016/j.ijbiomac.2019.01.063
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发表时间：
2019-04-15
影响因子：
8.2
通讯作者：
Guo, Yadong
中科院分区：
文献类型：
--
作者：
Ren, Lipin;Shang, Yanjie;Guo, Yadong
家蝇科，通常被称为家蝇及其近亲，是公认的医学，兽医和生态重要性的双翅目昆虫之一。线粒体基因组因其难以在形态上区分而被广泛应用于系统发育分析和分类学诊断。在这项研究中，我们的完整的有丝分裂基因组进行了测序和比对，其长度范围从15,117 bp（裸斑合成蝇）到16,089 bp（家蝇），并包含一个典型的环状分子，包括13个蛋白质编码基因（PCG），两个核糖体RNA（rRNA），22个转移RNA（tRNA）和一个非编码控制区。基因的排列顺序和方向与祖先昆虫一致。基于线粒体基因的系统发育分析表明，家蝇亚科内的亚科关系为（Mydaeinae（Muscinae（Reinwardtiinae + Azeliinae）。从最大似然（ML）和贝叶斯推断（BI）分析中恢复了相似的树拓扑结构。此外，我们比较了使用内部转录间隔区2（1752），延伸因子-1 α构建的系统发育分析。(EF-1 13例PCG和13例PCG +1752 + EF-1 α。核基因分区的组合分析提高了拓扑结构的支持度和分辨率，但由于物种有限，分支的位置明显不一致。更多的有丝分裂基因组序列的测定，尤其是近缘种的有丝分裂基因组序列的测定，将有助于我们对蝇类系统发育关系的认识。(C)2019爱思唯尔B.V.保留所有权利。
Muscidae, commonly known as house flies and their close relatives, is one of the dipteran insects of recognized medical, veterinary, and ecological importance. Mitochondrial genomes (Mitogenomes) have been widely used for exploring phylogenetic analysis and taxonomic diagnosis due to the difficulty in distinguishing them morphologically. In this study, our complete mitogenomes of muscid flies were sequenced and aligned, which ranged from 15,117 bp (Synthesiomyia nudiseta) to 16,089 bp (Musca sorbens) in length, and contained a typical circular molecule comprising 13 protein-coding genes (PCGs), two ribosomal RNAs (rRNAs), 22 transfer RNAs (tRNAs) and a non-coding control region. The order and orientation of genes were identical with that from the ancestral insects. The phylogenetic analysis based on the mitochondrial genes indicated that the subfamily relationships within Muscidae were reconstructed as (Mydaeinae (Muscinae (Reinwardtiinae + Azeliinae))). Similar tree topologies were recovered from both Maximum Likelihood (ML) and Bayesian Inference (BI) analysis. Furthermore, we compared the phylogenetic analyses that were constructed using internal transcribed spacer 2 (1752), elongation factor-1 alpha. (EF-1 alpha), 13 PCGs and 13 PCGs + 1752 + EF-1 alpha, respectively. Combined analysis of nuclear gene partitions improved support and resolution for resulting topologies but the positions of branches were obviously inconsistent due to limited species. More mitogenomes should be sequenced representing various taxonomic levels, especially close related species, which will enhance our understanding of phylogenetic relationships among muscids. (C) 2019 Elsevier B.V. All rights reserved.