Survival of Vibrio alginolyticus in seawater and retention of virulence of its starved cells
Survival of Vibrio alginolyticus in seawater and retention of virulence of its starved cells
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DOI：
10.1016/j.marenvres.2007.04.002
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发表时间：
2007-10-01
影响因子：
3.3
通讯作者：
Bakhrouf, Amina
中科院分区：
文献类型：
--
作者：
Kahla-Nakbi, Arnel Ben;Besbes, Amine;Bakhrouf, Amina
本研究的目的是评价4株溶藻弧菌在饥饿条件下在海水中的生存反应。我们使用含有消毒海水的微型宇宙，并在环境温度（22-25摄氏度）下孵育。溶藻弧菌的培养能力至少维持了9个月。长期饥饿的细胞表现出其酶活性的缺失或降低。对有活力但不可培养（VBNC）细胞进行复苏试验，并在添加营养物质后实现这些细胞的恢复。扩增16S核糖体DNA （rDNA）限制性内切分析（ARDRA）证实，在所有微生物中，同一株溶藻酶V在很长一段时间内持续存在。饥饿细胞对金头鲷（Sparus aurata）和黑鲈（Dicentrachus labrax）的感染通过腹腔刺激得以维持。(c) 2007 Elsevier Ltd.版权所有。
The aim of this study was to evaluate the survival responses of four strains of Vibrio alginolyticits in seawater under starvation conditions. We used microcosms containing sterilised seawater and incubated at ambient temperature (22-25 degrees C). V alginolyticus maintained its culturability for at least nine months. Long-term-starved cells showed an absence or a decrease in their enzymatic activities. Resuscitation assays of viable but non-culturable (VBNC) cells were conducted and the recovery of these cells was achieved after the addition of nutrients. Amplified 16S ribosomal DNA (rDNA) restriction analysis (ARDRA) was used to confirm that the same strain of V alginolyticus persisted in all microcosms during a long period of time. Starved cells maintained their infectivity for gilt head sea bream (Sparus aurata) and sea bass (Dicentrachus labrax) as determined by intraperitoneal challenges. (c) 2007 Elsevier Ltd. All rights reserved.