ANTIBODIES TO POLYSIALIC ACID AND ITS N-PROPYL DERIVATIVE - FINDING PROPERTIES AND INTERACTION WITH HUMAN EMBRYONAL BRAIN GLYCOPEPTIDES
ANTIBODIES TO POLYSIALIC ACID AND ITS N-PROPYL DERIVATIVE - FINDING PROPERTIES AND INTERACTION WITH HUMAN EMBRYONAL BRAIN GLYCOPEPTIDES
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DOI：
10.1093/infdis/171.6.1481
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发表时间：
1995-06-01
影响因子：
6.4
通讯作者：
FINNE, J
中科院分区：
文献类型：
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作者：
HAYRINEN, J;JENNINGS, H;FINNE, J
由于宿主组织聚唾液酸与荚膜多糖交叉反应诱导耐受性，因此没有针对B群脑膜炎球菌性脑膜炎的有效疫苗。使用配体结合试验研究了聚唾液酸-抗体相互作用的特异性。抗体735、20-1、2- 1 B、2- 2 B、5 E1和t5 E1以及针对N-丙酰化的B群脑膜炎球菌多糖-破伤风环形结合物的抗体（NP-4、106-6）结合多聚唾液酸化的人胚脑糖肽，但不结合对照糖肽或二唾液酰乳糖，而抗体109-3和I-627对N-丙酰化多糖更具特异性。抗神经节苷脂抗体（KM 538，KM 641）与聚唾液酸无交叉反应。人类类别转换抗体5E 1（IgM）和t5 E1（IgG）与所有测试化合物的反应相同，未观察到温度依赖性差异。所有抗聚唾液酸抗体需要8-10个残基的多糖链用于结合，而与免疫抗原、动物物种或免疫球蛋白类别无关。结果表明，在疫苗开发中应仔细评估聚唾液酸的交叉反应性。
There is no efficient vaccine against group B meningococcal meningitis because of tolerance induced by host tissue polysialic acid cross-reacting with the capsular polysaccharide. The specificities of polysialic acid-antibody interactions were studied using a ligand binding assay. Antibodies 735, 20-1, 2-1B, 2-2B, 5E1, and t5E1 and antibodies against N-propionylated group B meningococcal polysaccharide-tetanus toroid conjugate (NP-4, 106-6) bound polysialylated human embryonal brain glycopeptides but not control glycopeptides or disialosyllactose, whereas antibodies 109-3 and I-627 were more specific for the N-propionylated polysaccharide. Antiganglioside antibodies (KM538, KM641) did not cross-react with polysialic acid. Human class-switched antibodies 5E1 (IgM) and t5E1 (IgG) reacted identically with all compounds tested and no temperature-dependent differences were observed. All anti-polysialosyl antibodies required a polysaccharide chain of 8-10 residues for binding independent of the immunizing antigen, animal species, or immunoglobulin class. The results suggest careful evaluation of polysialic acid cross-reactivity in vaccine development.