Expression of HMP/AN2, a melanoma associated antigen, in murine cerebral gliomas: potential for radioimmunotargeting.
Expression of HMP/AN2, a melanoma associated antigen, in murine cerebral gliomas: potential for radioimmunotargeting.
复制标题
HMP/AN2（一种黑色素瘤相关抗原）在小鼠脑胶质瘤中的表达：放射免疫靶向的潜力。
DOI：
10.1007/s11060-009-9798-3
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发表时间：
2009
影响因子：
3.9
通讯作者：
Fenstermaker,RobertA
中科院分区：
文献类型：
--
作者：
Paul,AsitK;Ciesielski,MichaelJ;Sajjad,Munawwar;Wang,Xinhui;Ferrone,Soldano;Abdel-Nabi,Hani;Fenstermaker,RobertA
人黑色素瘤蛋白聚糖（HMP）是一种黑色素瘤相关抗原，在人黑色素瘤和神经胶质瘤中均有表达。我们使用HMP特异性单克隆抗体（mAb）VT 68.2来研究小鼠GL 261脑胶质瘤是否表达HMP同源物AN 2，并确定AN 2是否可以作为选择性体内放射治疗的靶向。HMP特异性mAb VT 68.2染色在同系C57 BL/6小鼠中培养和脑内生长的鼠GL 261胶质瘤细胞。正电子发射断层扫描与放射性标记的单克隆抗体VT 68.2显示高对比度，阳性图像的胶质瘤对阴性背景。在注射后96小时，放射性标记的mAb VT 68.2的胶质瘤摄取是同种型对照mAb的10倍，是正常脑组织中检测到的20倍。我们的研究结果表明，小鼠GL 261脑胶质瘤表达AN 2和HMP特异性mAb VT 68.2选择性地和特异性地积累在高浓度，并保留在小鼠脑胶质瘤内。因此，HMP是一个潜在的目标抗体介导的选择性递送放射治疗脑胶质瘤在体内。
Human melanoma proteoglycan (HMP), a melanoma-associated antigen, is expressed in both human melanomas and gliomas. We used HMP-specific monoclonal antibody (mAb) VT68.2 to investigate whether murine GL261 cerebral gliomas express the HMP homologue AN2 and to determine whether AN2 could be targeted for selective delivery of radiation in vivo. HMP-specific mAb VT68.2 stained murine GL261 glioma cells grown in culture and intracerebrally in syngeneic C57BL/6 mice. Positron emission tomography with radiolabeled mAb VT68.2 showed high-contrast, positive images of gliomas against a negative background. At 96 h after injection, glioma uptake of radiolabeled mAb VT68.2 was 10× greater than that of the isotype control mAb and 20× greater than that detected in normal cerebral tissue. Our results show murine GL261 cerebral gliomas express AN2 and HMP-specific mAb VT68.2 accumulates selectively and specifically at high concentration and is retained within murine cerebral gliomas. Thus, HMP is a potential target for antibody-mediated selective delivery of radiation to cerebral gliomas in vivo.