Resolving the resolution of array CGH
Resolving the resolution of array CGH
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DOI：
10.1016/j.ygeno.2006.12.012
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发表时间：
2007-05-01
期刊：
影响因子：
4.4
通讯作者：
Lam, Wan L.
中科院分区：
文献类型：
--
作者：
Coe, Bradley P.;Ylstra, Bauke;Lam, Wan L.
许多最近的技术已经被设计来取代传统的中期CGH技术，其目标是改进整个基因组中片段拷贝数状态的描述。然而，新技术的出现导致了如何充分描述每个阵列平台的功能的混乱。CGH阵列的设计可以包括均匀或高度可变的元件分布。这可能会导致平均或中位数分辨率的报告存在偏差，从而难以对平台进行公平比较。在这份报告中，我们提出了一个新的定义的阵列计算全息图技术的分辨率，称为“功能分辨率”，它结合了阵列上的元素间距的均匀性，以及每个平台的灵敏度单拷贝更改。通过开发适用于任何阵列CGH平台的基于Java的应用程序“ResCalc”，可以自动计算这些指标。(c)2007年爱思唯尔公司All rights reserved.
Many recent technologies have been designed to supplant conventional metaphase CGH technology with the goal of refining the description of segmental copy number status throughout the genome. However, the emergence of new technologies has led to confusion as to how to describe adequately the capabilities of each array platform. The design of a CGH array can incorporate a uniform or a highly variable element distribution. This can lead to bias in the reporting of average or median resolutions, making it difficult to provide a fair comparison of platforms. In this report, we propose a new definition of resolution for array CGH technology, termed "functional resolution," that incorporates the uniformity of element spacing on the array, as well as the sensitivity of each platform to single-copy alterations. Calculation of these metrics is automated through the development of a Java-based application, "ResCalc," which is applicable to any array CGH platform. (c) 2007 Elsevier Inc. All rights reserved.