Basophils are dispensable for the recovery of gross locomotion after spinal cord hemisection injury
Basophils are dispensable for the recovery of gross locomotion after spinal cord hemisection injury
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DOI：
10.1189/jlb.3ab0815-370r
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发表时间：
2016-04-01
影响因子：
5.5
通讯作者：
Hendrix, Sven
中科院分区：
文献类型：
--
作者：
Geurts, Nathalie;Vangansewinkel, Tim;Hendrix, Sven
嗜碱性粒细胞是循环系统中发现的最小粒细胞群。它们在过敏性疾病、预防寄生虫感染、自身免疫和 2 型免疫调节中发挥着重要且非冗余的作用。它们与肥大细胞具有相同的表型和功能特性，肥大细胞在脑外伤和脊髓损伤后发挥显着的保护作用，尽管它们被认为是体内最促炎的细胞类型之一。相比之下，嗜碱性粒细胞在中枢神经系统创伤中的体内功能仍然模糊且没有得到充分研究。在这项研究中，我们通过比较野生型与缺乏嗜碱性粒细胞的 Mcpt8Cre 转基因小鼠的脊髓损伤，发现嗜碱性粒细胞耗尽的小鼠的运动恢复不受影响。此外，两种小鼠品系之间的病变大小以及星形胶质细胞和巨噬细胞/小胶质细胞反应没有观察到显着差异。因此，尽管与肥大细胞共有多种特性，但据我们所知，这些数据首次表明，嗜碱性粒细胞对于小鼠脊髓半切损伤后的功能恢复过程是可有可无的。
Basophils are the smallest population of granulocytes found in the circulation. They have crucial and non-redundant roles in allergic disorders, in protection from parasite infections, in autoimmunity, and in the regulation of type 2 immunity. They share phenotypic and functional properties with mast cells, which exert substantial protective effects after traumatic brain injury and spinal cord injury, although they are considered one of the most proinflammatory cell types in the body. In contrast, the in vivo functions of basophils in central nervous system trauma are still obscure and not well studied. In this study, we show that by comparing spinal cord injury in wild type vs. basophil-deficient Mcpt8Cre transgenic mice, the locomotor recovery is not affected in mice depleted in basophils. In addition, no substantial differences were observed in the lesion size and in the astrocytic and macrophage/microglia reaction between both mouse strains. Hence, despite the multiple properties shared with mast cells, these data show, for the first time, to our knowledge, that basophils are dispensable for the functional recovery process after hemisection injury to the spinal cord in mice.