Expression profiles of non-small cell lung cancers on cDNA microarrays: Identification of genes for prediction of lymph-node metastasis and sensitivity to anti-cancer drugs
Expression profiles of non-small cell lung cancers on cDNA microarrays: Identification of genes for prediction of lymph-node metastasis and sensitivity to anti-cancer drugs
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DOI：
10.1038/sj.onc.1206288
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发表时间：
2003-04-10
期刊：
影响因子：
8
通讯作者：
Nakamura, Y
中科院分区：
文献类型：
--
作者：
Kikuchi, T;Daigo, Y;Nakamura, Y
为了研究涉及肺癌的基因以及与非原子癌对治疗药物的敏感性相关的基因，我们对来自原发性肿瘤的癌细胞的激光捕获微量解析后进行了37个NSCLC的cDNA微阵列分析。一种应用于表达数据的聚类算法很容易区分了两种主要的组织学类型的非小细胞肺癌，腺癌和鳞状细胞癌。随后对18种腺癌的分析确定了40个基因，其表达水平可以用淋巴结转移与无转移的病例分开。此外，我们将表达数据与手术解剖的NSCLC标本的敏感性的测量结果进行了比较嵌入式培养物敏感性测试）方法。我们发现数十个基因的表达水平与NSCLC的化学敏感性之间存在显着关联。我们的结果提供了有价值的信息，以最终确定这种类型的癌症的预测标记和新颖的治疗靶标分子。
To investigate genes involved in pulmonary carcinogenesis and those related to sensitivity of nonsmall cell lung cancers (NSCLCs) to therapeutic drugs, we performed cDNA microarray analysis of 37 NSCLCs after laser-capture microdissection of cancer cells from primary tumors. A clustering algorithm applied to the expression data easily distinguished two major histological types of non-small cell lung cancer, adenocarcinoma and squamous cell carcinoma. Subsequent analysis of the 18 adenocarcinomas identified 40 genes whose expression levels could separate cases with lymph-node metastasis from those without metastasis. In addition, we compared the expression data with measurements of the sensitivity of surgically dissected NSCLC specimens to six anti-cancer drugs (docetaxel, paclitaxel, irinotecan, cisplatin, gemcitabine, and vinorelbine), as measured by the CD-DST (collagen gel droplet embedded culture-drug sensitivity test) method. We found significant associations between expression levels of dozens of genes and chemosensitivity of NSCLCs. Our results provide valuable information for eventually identifying predictive markers and novel therapeutic target molecules for this type of cancer.