Deriving Extracellular Vesicle Size From Scatter Intensities Measured by Flow Cytometry.
Deriving Extracellular Vesicle Size From Scatter Intensities Measured by Flow Cytometry.
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DOI：
10.1002/cpcy.43
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发表时间：
2018-10-01
影响因子：
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通讯作者：
van der Pol, Edwin
中科院分区：
文献类型：
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作者：
de Rond, Leonie;Coumans, Frank A W;van der Pol, Edwin
流式细胞术通常用于研究细胞外囊泡（EV）作为疾病生物标志物的潜力。典型的流式细胞仪以任意单位检测单个EV的荧光和散射强度。这些任意单位使不同流式细胞仪之间的数据解释和数据比较复杂化。例如，检测到的EV浓度的比较需要了解可检测的EV大小。使用Mie理论和流式细胞仪的光学配置的知识，可以从给定EV折射率的散射强度导出EV尺寸。这里，描述了一种方案，以从散射强度导出EV和其他纳米颗粒的尺寸。由此产生的尺寸分布允许流式细胞仪之间的数据比较，这是EV作为生物标志物的临床应用的先决条件，并且可以推进纳米颗粒尺寸至关重要的其他领域。John Wiley & Sons，Inc.
Flow cytometry is commonly used to investigate the potential for extracellular vesicles (EVs) to be biomarkers of disease. A typical flow cytometer detects fluorescence and scatter intensities of single EVs in arbitrary units. These arbitrary units complicate data interpretation and data comparison between different flow cytometers. For example, comparison of detected EV concentrations requires knowledge of the detectable EV sizes. Using Mie theory and knowledge of the optical configuration of the flow cytometer, EV size can be derived from the scatter intensity for a given EV refractive index. Here, a protocol is described to derive the size of EVs and other nanoparticles from the scatter intensity. The resulting size distribution allows the comparison of data between flow cytometers, which is a prerequisite for clinical application of EVs as biomarkers and may advance other fields where sizing of nanoparticles is essential. © 2018 by John Wiley & Sons, Inc.