A targeted therapy for melanoma by graphene oxide composite with microRNA carrier.
A targeted therapy for melanoma by graphene oxide composite with microRNA carrier.
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DOI：
10.2147/dddt.s160088
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Zhou J
中科院分区：
文献类型：
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作者：
Liu C;Xie H;Yu J;Chen X;Tang S;Sun L;Chen X;Peng D;Zhang X;Zhou J
如今，microRNA (miR) 的组合在临床癌症试验中越来越受到关注。然而，由于 miR 的某些特性，如不稳定、低特异性分布和代谢毒性，其临床应用受到高度限制。为了提高miR治疗黑色素瘤的抗肿瘤功效并减少其副作用，本研究采用固体分散法制备了氧化石墨烯（GO）、壳聚糖（CS）和细胞穿透肽MPG的组合物。该研究通过表征研究分析了纳米载药复合物GO-CS和GO-CS-MPG的具体成分，证实了载体材料GO-CS-MPG的生物安全性。成功构建GO-CS-MPG-miR33a/miR199a纳米载药复合物并验证其药效。通过皮下肿瘤植入实验，证明载药复合物具有明显的抑制黑色素瘤细胞的作用。结果表明 GO-CS-MPG 可能在黑色素瘤治疗中具有潜在的应用。
Nowadays, the combination of microRNA (miR) is attracting increased attention in clinical cancer trials. However, the clinical use of miR is highly limited because of certain properties such as instability, low-specificity distribution, and metabolic toxicity. In order to improve the anti-tumor efficacy and reduce the side effects of miR in treating melanoma, a combination of graphene oxide (GO), chitosan (CS), and a cellular penetrating peptide, MPG, was prepared with solid dispersion method in this research. The research has analyzed the specific components of nano drug-loading complexes GO-CS and GO-CS-MPG through characterization research and confirmed the bio-safety of the carrier material GO-CS-MPG. The GO-CS-MPG-miR33a/miR199a nano drug-loading complex was successfully constructed and its medical effectiveness was verified. Through the subcutaneous tumor implantation experiment, an evident effect of the drug-loading complex in inhibiting melanoma cells was proven. Results suggest that GO-CS-MPG may have potential applications in melanoma therapy.