Fermentation study on Saccharina latissima for bioethanol production considering variable pre-treatments
Fermentation study on Saccharina latissima for bioethanol production considering variable pre-treatments
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DOI：
10.1007/s10811-008-9384-7
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发表时间：
2009-10-01
影响因子：
3.3
通讯作者：
Donnison, Iain S.
中科院分区：
文献类型：
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作者：
Adams, Jessica M.;Gallagher, Joseph A.;Donnison, Iain S.
气候变化、燃料安全和经济问题正在催生对可替代可再生燃料的需求。生物乙醇目前是由陆地作物生产的，但在未来，海洋生物质可以作为生物乙醇生产的替代生物质来源。像Laminaria spp.这样的大型藻类在英国周围大量生长，长度达到40米，含有高达55%的碳水化合物laminarin和甘露醇干重。利用酶，这些可以被水解并转化为葡萄糖和果糖，而葡萄糖和果糖又可以被酵母利用来生产乙醇。在以往的大型藻类乙醇生产研究中，发酵前的预处理是在65℃，pH 2下进行1小时。本文表明，这些预处理不需要进行发酵，未经处理的发酵比改变pH或温度预处理的发酵获得更高的乙醇产量。这一结果在使用酿酒酵母和1 U kg（-1）层状酶的新鲜和解冻的大型藻类样品中可见。
Climate change, fuel security and economics are creating a requirement for alternative, renewable fuels. Bioethanol is currently produced from land-based crops but in future marine biomass could be used as an alternative biomass source for bioethanol production. Macroalgae such as Laminaria spp. grow in abundance around the United Kingdom, reaching >4 m in length and containing up to 55% dry weight of the carbohydrates laminarin and mannitol. Using enzymes, these can be hydrolysed and converted to glucose and fructose, which in turn can be utilised by yeasts to produce ethanol. In previous studies on ethanol production from macroalgae, pre-treatment was at 65 C, pH 2 for 1 h prior to fermentation. This paper shows that these pre-treatments are not required for the fermentations conducted, with higher ethanol yields being achieved in untreated fermentations than in those with altered pH or temperature pre-treatments. This result was seen in fresh and defrosted macroalgae samples using Saccharomyces cerevisiae and 1 U kg(-1) laminarinase.