Evolution of complexity in the zebrafish synapse proteome.
Evolution of complexity in the zebrafish synapse proteome.
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DOI：
10.1038/ncomms14613
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发表时间：
2017-03-02
影响因子：
16.6
通讯作者：
Grant SG
中科院分区：
文献类型：
--
作者：
Bayés À;Collins MO;Reig-Viader R;Gou G;Goulding D;Izquierdo A;Choudhary JS;Emes RD;Grant SG
人脑突触的蛋白质组非常复杂，在130多种疾病中发生突变。这种复杂性源于脊椎动物谱系早期的两个全基因组复制。斑马鱼用于模拟人类疾病;然而，其突触蛋白质组是未表征的，并且硬骨鱼特异性基因组复制（TSGD）是否影响复杂性尚不清楚。本文报道了斑马鱼中枢突触的蛋白质组和超微结构特征，并分析了TSGD的重要性。虽然TSGD增加了整体突触蛋白质组的复杂性，但斑马鱼的突触后密度（PSD）蛋白质组的复杂性低于哺乳动物。一组高度保守的1,000种蛋白质在脊椎动物中是共有的。PSD超微结构特征也是保守的。谱系特异性蛋白质组差异表明脊椎动物物种进化出不同的突触类型和功能。这些数据集是一个广泛的研究资源，并对使用斑马鱼模拟人类突触疾病具有重要意义。对斑马鱼突触蛋白质组的系统分析一直缺乏。在本文中，作者描述了斑马鱼突触的超微结构，并比较了斑马鱼和小鼠突触后密度的蛋白质组，为将来利用斑马鱼进行疾病模型研究提供了资源。
The proteome of human brain synapses is highly complex and is mutated in over 130 diseases. This complexity arose from two whole-genome duplications early in the vertebrate lineage. Zebrafish are used in modelling human diseases; however, its synapse proteome is uncharacterized, and whether the teleost-specific genome duplication (TSGD) influenced complexity is unknown. We report the characterization of the proteomes and ultrastructure of central synapses in zebrafish and analyse the importance of the TSGD. While the TSGD increases overall synapse proteome complexity, the postsynaptic density (PSD) proteome of zebrafish has lower complexity than mammals. A highly conserved set of ∼1,000 proteins is shared across vertebrates. PSD ultrastructural features are also conserved. Lineage-specific proteome differences indicate that vertebrate species evolved distinct synapse types and functions. The data sets are a resource for a wide range of studies and have important implications for the use of zebrafish in modelling human synaptic diseases. Systematic analysis of the zebrafish synapse proteome has been lacking. Here the authors characterize the ultrastructure of zebrafish synapse and compare the proteomes of postsynaptic density in zebrafish and mice, offering a resource for future studies using zebrafish to model diseases.