Heterologous expression of three stress-responsive genes from Nostoc flagelliforme confers tolerance to abiotic stresses in Escherichia coli
Heterologous expression of three stress-responsive genes from Nostoc flagelliforme confers tolerance to abiotic stresses in Escherichia coli
复制标题
发菜中三个胁迫响应基因的异源表达赋予大肠杆菌对非生物胁迫的耐受性
DOI：
10.1007/s10811-013-0064-x
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发表时间：
2014-02-01
影响因子：
3.3
通讯作者：
Qiu, Baosheng
中科院分区：
文献类型：
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作者：
Ai, Yufeng;Yang, Yiwen;Qiu, Baosheng
发状念珠藻是一种陆地抗旱蓝细菌，其潜在的遗传资源尚未得到充分开发。在这项研究中，我们评估了三个候选基因drnf3，5和9的潜在压力调节相关功能。转录分析表明，这三个基因是应激反应。通过将它们导入表达载体pET 28a和大肠杆菌菌株BL 21中，对其功能进行了评价。当与用空载体转化的对照菌株相比时，三种转化菌株中的每一种在渗透和盐胁迫条件下表现出增强的细胞生长。三个转化菌株在正常条件下也显示出增强的细胞生长。这些结果表明，提高非生物胁迫抗性可能与这三个基因在促进细胞生长的基本功能。其实际的胁迫调节分子机制仍有待探索。然而，从应用的角度来看，它们将作为重要的候选基因，在发展的转基因工业菌株，需要耐渗透或盐胁迫。
Nostoc flagelliforme is a terrestrial drought-resistant cyanobacterium whose potential genetic resources remain poorly explored. In this study, we evaluated the potential stress regulation-associated functions of three candidate genes, drnf3, 5, and 9. Transcriptional analysis showed that these three genes were stress responsive. Their functions were evaluated by introducing them into the expression vector pET28a and Escherichia coli strain BL21. Each of the three transformed strains exhibited enhanced cell growth in osmotic and salt stress conditions when compared to a control strain transformed with the empty vector. Three transformed strains also showed enhanced cell growth under normal condition. These results imply that increased abiotic stress resistance may be related to the basal functions of these three genes in promoting cell growth. Their actual molecular mechanisms of stress regulation remain to be explored. However, from an applied point of view, they would serve as important candidate genes in the development of transgenic industrial strains that need to be resistant to osmotic or salt stress.