Fourier transform ion cyclotron resonance mass spectrometric analysis of the green fraction of soil humic acids.
Fourier transform ion cyclotron resonance mass spectrometric analysis of the green fraction of soil humic acids.
复制标题
土壤腐植酸绿色部分的傅里叶变换离子回旋共振质谱分析。
DOI：
10.1002/rcm.6718
复制
发表时间：
2013
期刊：
影响因子：
--
通讯作者：
A. Watanabe
中科院分区：
文献类型：
--
作者：
K. Ikeya;R. Sleighter;P. Hatcher;A. Watanabe
理由
土壤腐殖酸（HA）的绿色部分（Pg）含有作为发色团的4，9-二羟基苝-3，10-醌（DHPQ）。虽然已知存在各种天然存在的DHPQ衍生物，并且在来自世界各地各种类型土壤的HA的紫外-可见光谱中观察到Pg样吸收，但Pg的化学结构仍然未知。
方法
为了更好地了解Pg的化学组成，预分离（粗Pg）和纯化的Pg（G2）样品和三个土壤腐殖化程度不同的腐殖酸进行了分析，使用负模式电喷雾电离耦合傅里叶变换离子回旋共振质谱（FTICRMS）在12特斯拉。使用H/C-O/C比率并基于具有DHPQ或相关化合物作为潜在结构的分子式的类型和大小来表征Pg样品。
结果
H/C-O/C图表明，大部分的脂质，蛋白质，其他淀粉，和木质素类成分被删除，而浓缩的芳香族成分的粗Pg到G2使用Sephadex G-50的纯化过程中。超过27个分子式类似DHPQ，DHPQ取代不同数量的羧基和/或羟基，和苯基-DHPQ衍生物中确定的两个Pg样品。这些公式在G_2中的峰值幅度（占总分配幅度的40%）大于在P_8粗品中的峰值幅度（占21%）。这27个分子式中的大多数也在3种土壤腐殖酸中检测到，表明在各种土壤腐殖酸中普遍存在Pg相关化合物。
结论
利用FTICRMS对Pg组分的重要分子式进行了估算。版权所有© 2013约翰威利父子有限公司.
RATIONALE
The green fraction of soil humic acids (HAs), designated Pg, contains 4,9-dihydroxyperylene-3,10-quinone (DHPQ) as a chromophore. Although various naturally occurring DHPQ derivatives are known to occur and Pg-like absorption is observed in the UV-visible spectra of HAs from various types of soils worldwide, the chemical structure of Pg is still unknown.
METHODS
For a better understanding of the chemical composition of Pg, the pre-isolated (crude Pg) and purified Pg (G2) samples and three soil HAs with different degrees of humification were analyzed using negative-mode electrospray ionization coupled with Fourier transform ion cyclotron resonance mass spectrometry (FTICRMS) at 12 Tesla. The Pg samples were characterized using H/C-O/C ratios and based on the type and magnitude of the molecular formulae having DHPQ or related compounds as a potential structure.
RESULTS
The H/C-O/C diagram indicated that most of the lipids, proteins, other aliphatics, and lignin-like components were removed, while condensed aromatic components were concentrated during the purification process of crude Pg to G2 using Sephadex G-50. More than 27 molecular formulae resembling DHPQ, DHPQ substituted with varying numbers of carboxyl and/or hydroxyl groups, and phenyl-DHPQ derivatives were identified in both Pg samples. The peak magnitudes of these formulae in G2 (accounting for 40% of the total assigned magnitude) were greater than that in the crude Pg (21%). Most of the 27 molecular formulae were also detected in the three soil HAs, suggesting a common existence of Pg-related compounds in various soil HAs.
CONCLUSIONS
The important molecular formulae of Pg components were estimated utilizing FTICRMS. Copyright © 2013 John Wiley & Sons, Ltd.
利用四氧化钌氧化分析土壤腐植酸中的多核芳香族和脂肪族成分
DOI：
--
发表时间：
2007
期刊：
Eur.J.Soil Sci., 58
影响因子：
--
作者：
K.Ikeya;H.Ohtani;et. al.
通讯作者：
et. al.