Roles of differential expression of microRNA-21-3p and microRNA-433 in FSH regulation in rat anterior pituitary cells.
Roles of differential expression of microRNA-21-3p and microRNA-433 in FSH regulation in rat anterior pituitary cells.
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microRNA-21-3p和microRNA-433差异表达在大鼠垂体前叶细胞FSH调节中的作用
DOI：
10.18632/oncotarget.16615
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发表时间：
2017-05-30
期刊：
影响因子：
--
通讯作者：
Zhang JB
中科院分区：
文献类型：
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作者：
Han DX;Sun XL;Xu MQ;Chen CZ;Jiang H;Gao Y;Yuan B;Zhang JB
腺垂体细胞分泌的促卵泡激素（FSH）在生殖调控中起重要作用，但microRNA（miRNAs）是否调控FSH的分泌尚不清楚。在本研究中，我们使用TargetScan程序和荧光素酶报告基因分析来预测和筛选可能作用于大鼠卵泡刺激素β亚基（FSHb）基因的miRNAs，结果鉴定了两种miRNAs，miR-21- 3 p和miR-433。然后将这些miRNAs转染到大鼠前腺垂体细胞中，通过定量PCR和ELISA评估转染细胞中FSHb表达水平和FSH分泌。结果表明，与对照组相比，miR-21- 3 p和miR-433均下调FSHb的表达水平，导致FSH分泌减少; miR-21- 3 p和miR-433抑制剂处理后，FSHb的表达水平上调，FSH分泌增加。综上所述，我们的研究结果表明miR-21- 3 p和miR-433可以通过直接靶向大鼠原代垂体细胞FSHb 3′UTR下调FSHb的表达。我们的研究结果提供了证据表明，miRNAs可以调节FSHb的表达，并进一步影响FSH的分泌，并可能有助于使用miRNAs调节动物生殖。
Follicle-stimulating hormone (FSH) secreted by adenohypophyseal cells plays an important role in the regulation of reproduction, but whether microRNAs (miRNAs) regulate the secretion of FSH remains unclear. In the present study, we predicted and screened miRNAs that might act on the follicle-stimulating hormone beta-subunit (FSHb) gene of rats using the TargetScan program and luciferase reporter assays, and the results identified two miRNAs, miR-21-3p and miR-433. We then transfected these miRNAs into rat anterior adenohypophyseal cells and assessed the FSHb expression levels in and FSH secretion by the transfected cells through quantitative PCR and ELISA. The results showed that both miR-21-3p and miR-433 down-regulated the expression levels of FSHb and resulted in the decrease of the secretion of FSH compared with the control group, and treatment with miR-21-3p and miR-433 inhibitors up-regulated the expression levels of FSHb and resulted in the increase of the secretion of FSH. Taken together, our results indicate that miR-21-3p and miR-433 can down-regulate the expression of FSHb by directly targeting the FSHb 3′UTR in rat primary pituitary cells. Our findings provide evidence that miRNAs can regulate FSHb expression and further affect the secretion of FSH and might contribute to the use of miRNAs for the regulation of animal reproduction.