ESTIMATION OF PRODUCT OF LIPID PEROZIDATION (MALONYL DIALDEHYDE) IN BIOCHEMICAL SYSTEMS
ESTIMATION OF PRODUCT OF LIPID PEROZIDATION (MALONYL DIALDEHYDE) IN BIOCHEMICAL SYSTEMS
复制标题
DOI：
10.1016/0003-2697(66)90167-9
复制
发表时间：
1966-01-01
影响因子：
2.9
通讯作者：
JOHNSON, BC
中科院分区：
文献类型：
--
作者：
PLACER, ZA;CUSHMAN, LL;JOHNSON, BC
描述了一种新的硫代巴比妥酸方法，用于估计由于脂质过氧化而产生的丙二酰二醛，其中在碱性溶液中估计三甲川有色物质。碱性吡啶-丁醇混合物可溶解反应过程中沉淀或乳白色的蛋白质和脂质，从而产生更准确的 MDA 估算并简化程序。
A new thiobarbituric acid method for the estimation of the malonyl dialdehyde produced as a result of lipid peroxidation is described in which the trimethine colored substance is estimated in alkaline solution. Alkaline pyridine-butanol mixture dissolves both proteins and lipids that are precipitated or opalescent during the reaction, yielding a more accurate MDA estimation as well as simplifying the procedure.