WISP-2 as a novel estrogen-responsive gene in human breast cancer cells.
WISP-2 as a novel estrogen-responsive gene in human breast cancer cells.
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DOI：
10.1006/bbrc.2000.3276
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发表时间：
2000-08
影响因子：
3.1
通讯作者：
Hidekuni Inadera;S. Hashimoto;Hong‐yan Dong;Takuji Suzuki;S. Nagai;T. Yamashita;Nobuaki Toyoda;K. Matsushima
中科院分区：
文献类型：
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作者：
Hidekuni Inadera;S. Hashimoto;Hong‐yan Dong;Takuji Suzuki;S. Nagai;T. Yamashita;Nobuaki Toyoda;K. Matsushima
为了寻找新的雌激素应答基因，我们对雌激素处理的MCF-7人乳腺癌细胞进行了基因表达系列分析（SAGE）。SAGE分析31，000和30，856标签从未处理的和17 β-雌二醇（E2）处理的细胞24小时，分别促进了15，037不同的转录本的鉴定。这两个SAGE文库的比较表明，在表达谱显着的相似性。在确定的成绩单，四个基因被发现显着增加E2处理的细胞与对照细胞相比。其中三个转录本是组织蛋白酶D、pS2和高迁移率族1蛋白，它们被描述为雌激素诱导基因。第四个基因是WISP-2（Wnt-1诱导信号通路蛋白2），其最近被报道为在由Wnt-1癌基因转化的乳腺上皮细胞系C57 MG中上调的基因。通过与纯抗雌激素ICI 182，780共孵育完全阻止WISP-2 mRNA的增加，但与放线菌酮共孵育不能阻止WISP-2 mRNA的增加，表明WISP-2直接受雌激素受体调节。WISP-2基因也可以通过环境雌激素如双酚A或壬基酚诱导表达。这项研究代表了第一个全面的基因表达分析雌激素治疗的人乳腺癌细胞。
In order to search for novel estrogen-responsive genes, we performed serial analysis of gene expression (SAGE) for estrogen-treated MCF-7 human breast cancer cells. SAGE analysis of 31,000 and 30,856 tags from non-treated and 17 beta-estradiol (E2)-treated cells for 24 h, respectively, facilitated the identification of 15,037 different transcripts. Comparison of these two SAGE libraries indicated a remarkable similarity in expression profiles. Among the identified transcripts, four genes were found to be markedly increased for E2-treated cells compared with control cells. Three of the transcripts were cathepsin D, pS2 and high mobility group 1 protein, which have been described as estrogen-inducible genes. The fourth gene was WISP-2 (Wnt-1 inducible signaling pathway protein 2) which has recently been reported as an up-regulated gene in the mammary epithelial cell line C57 MG transformed by the Wnt-1 oncogene. The increase in WISP-2 mRNA was completely prevented by co-incubation with a pure anti-estrogen ICI 182,780, but not by coincubation with cycloheximide, indicating that WISP-2 is directly regulated by the estrogen receptor. The WISP-2 gene was also induced by treating with environmental estrogens, such as bisphenol-A or nonylphenol. This study represents the first comprehensive gene expression analysis of estrogen-treated human breast cancer cells.