Stimulation of a cell-mediated cytotoxic response to FeLV-induced T cell lymphomas in the cat.
Stimulation of a cell-mediated cytotoxic response to FeLV-induced T cell lymphomas in the cat.
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刺激猫体内 FeLV 诱导的 T 细胞淋巴瘤的细胞介导的细胞毒性反应。
作者：
M. Tompkins;W. Tompkins
在正常和牛痘病毒感染的成年家猫的外周血和脾脏中检查了非特异性细胞介导的细胞毒性。通过裂解痘苗病毒或 HSV 感染的贴壁猫舌细胞来测量，在正常非免疫猫的脾脏和外周血中发现了天然细胞毒性 (NC) 样细胞。细胞毒性在 16 小时测定中表达，但在 4 小时测定中不表达。通过裂解悬浮生长的 FeLV 诱导的淋巴肿瘤细胞系 (FL-74) 来测量，在脾脏中发现了自然杀伤 (NK) 样细胞，但在 PBL 中未发现，并且需要进行 16 小时的表达测定。痘苗病毒感染不会增加猫外周血或脾脏中 NC 样细胞的活性。然而，NK 样功能有所增加。细胞毒性在感染后 6 天达到峰值，需要进行 16 小时测定才能获得细胞裂解的最大表达。此外，在牛痘感染后 6 天，循环中出现了一种具有脾脏 NK 样细胞细胞毒性特征的细胞，其水平较低。在 4 小时的测定中，来自痘苗病毒感染的猫的 NK 样细胞对 FL74 靶标表现出一定的细胞毒性。因此，猫拥有至少两种功能不同的天然细胞毒性细胞群。 NC 样细胞存在于脾脏和外周血中，裂解病毒感染的单层靶标，并且不会被感染激活。 NK 样细胞存在于脾脏、裂解性淋巴肿瘤靶标中，可通过感染激活，其峰值活性出现在感染后 6 天。
Nonspecific cell-mediated cytotoxicity was examined in the peripheral blood and spleens of normal and vaccinia virus-infected adult domestic cats. Natural cytotoxic (NC)-like cells, as measured by lysis of vaccinia- or HSV-infected, adherent cat tongue cells, were found in both the spleen and peripheral blood of normal, nonimmune cats. Cytotoxicity was expressed in a 16-hr assay but not in a 4-hr assay. Natural killer (NK)-like cells, as measured by lysis of an FeLV-induced lymphoid tumor cell line (FL-74) growing in suspension, were found in the spleen but not PBL, and required a 16-hr assay for expression. Infection with vaccinia virus did not increase the activity of feline NC-like cells in either the peripheral blood or the spleen. NK-like function, however, was increased. Cytotoxicity peaked 6 days post-infection and required a 16-hr assay for maximal expression of cell lysis. Furthermore, a cell with cytotoxic characteristics of the spleen NK-like cell appeared at low levels in the circulation at 6 days post-vaccinia infection. NK-like cells from vaccinia-infected cats showed some cytotoxicity for FL74 targets in a 4-hr assay. The cat thus possesses at least two functionally different populations of naturally cytotoxic cells. NC-like cells are found in the spleen and peripheral blood, lyse virus-infected monolayer targets, and are not activated by infection. NK-like cells are found in the spleen, lyse-lymphoid tumor targets, and can be activated by infection, with their peak activity occurring 6 days after infection.