Genetic characterization of Toxoplasma gondii isolates from China
Genetic characterization of Toxoplasma gondii isolates from China
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DOI：
10.1016/j.parint.2009.01.006
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发表时间：
2009-06-01
影响因子：
1.9
通讯作者：
Zhu, Xing-Quan
中科院分区：
文献类型：
--
作者：
Zhou, Peng;Zhang, Han;Zhu, Xing-Quan
弓形虫感染在世界范围内的人类和动物中普遍存在。在北美和欧洲，T.弓形虫是高度克隆的，由三个不同的谱系（I型、II型和III型）组成。而在南美洲，弓形虫具有高度多样性，在种群中有少数谱系扩展。然而，关于亚洲弓形虫多样性的数据有限。本文报道了应用多位点PCR-RFLP技术对我国不同宿主和地理分布的弓形虫分离株的遗传特征进行分析。共17个T。对人（3株）、羊（1株）、猪（5株）和猫（8株）弓形虫分离株进行了10个遗传标记分型，包括9个核位点SAG 1、SAG 2、SAG 3、BTUB、GRA 6、L358、PK 1、c22-8、c29-2和顶质体Apico位点。发现了4种基因型，包括3种以前报道的基因型和1种新的基因型。3个分离物属于克隆I型谱系，1个分离物属于克隆II型谱系，其余13个分离物分为两种基因型。这是首次利用多个遗传标记对我国不同宿主和地理分布的弓形虫分离株进行遗传分型，为弓形虫群体遗传结构的研究提供了依据。弓形虫，以及在中国预防和控制人和动物的弓形虫感染。(C)2009爱思唯尔爱尔兰有限公司保留所有权利。
Toxoplasma gondii infections are prevalent in humans and animals worldwide. In North America and Europe, T. gondii is highly clonal, consisting of three distinct lineages (Types I, II and III). whereas in South America, T gondii is highly diverse with a few lineages expanded in the population. However, there is limited data on the diversity of T gondii in Asia. Here we report the genetic characterization of T gondii isolates from different hosts and geographical locations in China using the multilocus PCR-RFLP. A total of 17 T. gondii isolates from humans (3 Strains), sheep (1 strain), pigs (5 strains) and cats (8 strains) were typed at 10 genetic markers including 9 nuclear loci SAG1, SAG2, SAG3, BTUB, GRA6, L358, PK1, c22-8, c29-2 and an apicoplast locus Apico. Four genotypes were revealed, including three previously reported and one new genotype. Three isolates belong to the clonal Type I lineage, one isolate belongs to the clonal Type II lineage, and the rest 13 isolates are grouped into two genotypes. This is the first report of genetic typing of T gondii isolates from different hosts and geographical locations in China using a number of genetic markers, which has implications for the studies of population genetic structures of T. gondii, as well as for the prevention and control of T gondii infections in humans and animals in China. (C) 2009 Elsevier Ireland Ltd. All rights reserved.