Engineering Peptide-Based Polyelectrolyte Complexes with Increased Hydrophobicity
Engineering Peptide-Based Polyelectrolyte Complexes with Increased Hydrophobicity
复制标题
工程设计具有增强疏水性的基于肽的聚电解质复合物
作者：
Sara Tabandeh;Lorraine Leon
聚电解质络合是设计自组装材料的通用平台。在这里，我们使用合理的设计来创建离子疏水图案化的肽，使我们能够精确地探索疏水性对静电自组装的作用。聚阳离子和聚阴离子的设计和合成的交替序列的d-和l-手性模式的赖氨酸或谷氨酸与甘氨酸，丙氨酸或亮氨酸，由于其增加的疏水性指数，分别。两个图案被认为是相反电荷的图案化肽;一个具有相等的残基的带电和不带电的氨基酸和其他增加的电荷密度。质谱，圆二色谱，H-和F-NMR光谱用于表征多肽。使用浊度测量，光学显微镜和红外光谱法，其特征在于使用的序列形成的聚电解质复合物（佩奇）。我们的研究结果表明，临界盐浓度，PEC稳定性的关键措施，增加电荷密度以及疏水性。此外，通过增加疏水性，形成的PEC的量随温度增加，与纯离子佩奇相反。最后，我们使用疏水染料评估了这些材料的包封行为。得出结论，包封率增加的疏水含量的复合物提供洞察未来的工作，这些材料的药物输送的应用。
Polyelectrolyte complexation is a versatile platform for the design of self-assembled materials. Here we use rational design to create ionic hydrophobically-patterned peptides that allow us to precisely explore the role of hydrophobicity on electrostatic self-assembly. Polycations and polyanions were designed and synthesized with an alternating sequence of d- and l-chiral patterns of lysine or glutamic acid with either glycine, alanine or leucine due to their increasing hydrophobicity index, respectively. Two motifs were considered for the oppositely charged patterned peptides; one with equal residues of charged and uncharged amino acids and the other with increased charge density. Mass spectroscopy, circular dichroism, H- and F-NMR spectroscopy were used to characterize the polypeptides. Polyelectrolyte complexes (PECs) formed using the sequences were characterized using turbidity measurements, optical microscopy and infrared spectroscopy. Our results show that the critical salt concentration, a key measure of PEC stability, increased with both increasing charge density as well as hydrophobicity. Furthermore, by increasing the hydrophobicity, the amount of PEC formed increased with temperature, contrary to purely ionic PECs. Lastly, we assessed the encapsulation behavior of these materials using a hydrophobic dye. Concluding that encapsulation efficiency increased with hydrophobic content of the complexes providing insight for future work on the application of these materials for drug delivery.
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