Biological identifications through DNA barcodes: the case of the Crustacea
Biological identifications through DNA barcodes: the case of the Crustacea
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DOI：
10.1139/f07-008
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发表时间：
2007-02-01
影响因子：
2.4
通讯作者：
Hebert, Paul D. N.
中科院分区：
文献类型：
--
作者：
Costa, Filipe O.;deWaard, Jeremy R.;Hebert, Paul D. N.
线粒体细胞色素c氧化酶I （COI）基因的650碱基对片段已被证明能够提供物种水平的鉴定，用于昆虫、鸟类和鱼类等动物的大型分类组合，但对于甲壳类亚门（节肢动物中最多样化的群体之一）却没有。在本研究中，我们测试了COI在这个群体中提供鉴定的能力，检查了分类等级中的两个不同水平-目和种。我们研究的第一阶段是基于对150个物种的分析，建立23个优势甲壳目的序列剖面，每个物种属于不同的科。COI氨基酸数据将这些分类群置于内聚的组合中，这些组合的隶属关系在阶、超阶和亚类水平上与目前公认的边界一致。随后，在十足目和水蚤属（枝角目）和Gammarus属（片足目）的代表中，研究了物种水平的分辨率。这些研究表明，同属物种之间的核苷酸序列差异水平是同一物种个体之间的19至48倍。我们认为COI条形码区域的序列变化对甲壳类生物的种类区分是非常有效的。
The ability of a 650 base pair section of the mitochondrial cytochrome c oxidase I (COI) gene to provide species-level identifications has been demonstrated for large taxonomic assemblages of animals such as insects, birds, and fishes, but not for the subphylum Crustacea, one of the most diverse groups of arthropods. In this study, we test the ability of COI to provide identifications in this group, examining two disparate levels in the taxonomic hierarchy - orders and species. The first phase of our study involved the development of a sequence profile for 23 dominant crustacean orders, based upon the analysis of 150 species, each belonging to a different family. The COI amino acid data placed these taxa into cohesive assemblages whose membership coincided with currently accepted boundaries at the order, superorder, and subclass levels. Species-level resolution was subsequently examined in an assemblage of Decapoda and in representatives of the genera Daphnia (Cladocera) and Gammarus (Amphipoda). These studies revealed that levels of nucleotide sequence divergence were from 19 to 48 times greater between congeneric species than between individuals of a species. We conclude that sequence variation in the COI barcode region will be very effective for discriminating species of Crustacea.