Recombinant curculin heterodimer, exhibits taste-modifying and sweet-tasting activities
Recombinant curculin heterodimer, exhibits taste-modifying and sweet-tasting activities
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DOI：
10.1016/j.febslet.2004.07.073
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发表时间：
2004-08-27
期刊：
影响因子：
3.5
通讯作者：
Kato, K
中科院分区：
文献类型：
--
作者：
Suzuki, M;Kurimoto, E;Kato, K
来自阔叶仙茅的仙茅素是一种独特的甜味蛋白质，具有甜味和调味活性。我们分离出一个基因，该基因编码与仙茅素高度同源的新型蛋白质。使用先前已知的仙茅素（称为仙茅素1）和新的仙茅素亚型（仙茅素2）的cDNA，我们通过大肠杆菌表达系统产生了一组同二聚体和异二聚体重组仙茅素。结果表明，甜味和味道修饰活性仅由仙茅素 1 和仙茅素 2 的异二聚体表现出来。 (C) 2004 年欧洲生化学会联合会。由 Elsevier B.V. 出版。保留所有权利。
Curculin from Curculigo latifolia is a unique sweet protein that exhibits both sweet-tasting and taste-modifying activities. We isolated a gene that encodes a novel protein highly homologous to curculin. Using cDNAs of the previously known curculin (designated as curculin1) and the novel curculin isoform (curculin2), we produced a panel of homodimeric and heterodimeric recombinant curculins by Escherichia coli expression systems. It was revealed that sweet-tasting and taste-modifying activities were exhibited solely by the heterodimer of curculin1 and curculin2. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.