Evaluation of the Relationship Between Bioactive Components in Seaweeds and Advanced Glycation End-Products Inhibitory Activities Using Principal Component Analysis
Evaluation of the Relationship Between Bioactive Components in Seaweeds and Advanced Glycation End-Products Inhibitory Activities Using Principal Component Analysis
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DOI：
10.1007/s11130-021-00908-5
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发表时间：
2021-07-19
影响因子：
4
通讯作者：
Lee, Sang-Hoon
中科院分区：
文献类型：
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作者：
Cho, Chi Heung;Youm, Ga Hee;Lee, Sang-Hoon
本研究全面研究了38种海藻70% （v/v）乙醇水提物的生物活性物质与抗糖基化活性之间的关系。用比色法测定了枸杞中总酚、总黄酮、缩合单宁等生物活性物质的含量。其中，双曲Ecklonia bicyclis、石杉叶、小木耳的总酚含量最高（255.75 mg GAE/g DW），总浓缩单宁含量最高（63.36 mg CE/g DW），总黄酮含量最高（85.26 mg CE/g DW）。通过晚期糖基化终产物（AGEs）形成、AGEs-胶原交联形成和AGEs-胶原交联断裂实验来评估SWEs的抗糖基化特性。与氨基胍和ALT-711（阳性对照）相比，褐藻对AGEs形成、AGEs-胶原交联以及AGEs-胶原交联断裂的抑制作用更为显著。主成分分析证实，ses中总酚和缩合单宁含量与AGEs的抑制形成性能和AGEs-胶原断交联活性密切相关。因此，海藻中的酚类物质和缩合单宁等生物活性物质可作为筛选化合物的预测指标，用于开发预防AGEs相关糖尿病并发症的治疗药物。此外，我们的研究结果表明，褐藻比绿藻和红藻含有更多的生物活性物质，可以作为AGE抑制剂和交联抑制剂作为预防和减轻ages相关糖尿病并发症的一种有前景的天然资源。
This study comprehensively presents the relationship between the bioactive substance of 70% (v/v) aqueous ethanol extract of 38 species of seaweeds (SWEs), and anti-glycation activities. The contents of bioactive substance of SWEs, such as total phenolic, total flavonoid and condensed tannins, were determined through a colorimetric analysis. Among the tested species, Ecklonia bicyclis, Ishige foliacea, and Cladophora urightiana var. minor had the highest amount of total phenolic (255.75 mg GAE/g DW), total condensed tannins (63.36 mg CE/g DW), and total flavonoid content (85.26 mg CE/g DW), respectively. Anti-glycation properties of SWEs were evaluated through advanced glycation end-products (AGEs) formation, AGEs-collagen cross-link formation, and AGEs-collagen cross-link breaking assay. Brown algae species exhibited a more prominent inhibitory activity on AGEs formation and AGEs-collagen cross-links, and the breaking of AGEs-collagen cross-links compared to that exhibited by aminoguanidine and ALT-711 (positive controls). Using principal component analysis, we confirmed that the AGEs formation inhibitory property and AGEs-collagen cross-links breaking activity were closely correlated with total phenolic and the condensed tannin contents contained in SWEs. Therefore, the bioactive substances such as phenolics and condensed tannins in seaweeds can be used as predictive indices in selecting compounds for the development of a therapeutic agent that prevents diabetic complications related to the AGEs. In addition, our results suggest that brown algae species, which contains more bioactive substances than green and red algae species, can be utilized as a promising natural resource for the prevention and alleviation of AGEs-related diabetic complications as AGE inhibitor and cross-links breaker.