HuC/D confocal imaging points to olfactory migratory cells as the first cell population that expresses a post-mitotic neuronal phenotype in the chick embryo
HuC/D confocal imaging points to olfactory migratory cells as the first cell population that expresses a post-mitotic neuronal phenotype in the chick embryo
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DOI：
10.1016/j.neuroscience.2003.07.004
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发表时间：
2003-01-01
期刊：
影响因子：
3.3
通讯作者：
Giacobini-Robecchi, MG
中科院分区：
文献类型：
--
作者：
Fornaro, M;Geuna, S;Giacobini-Robecchi, MG
在本研究中，HuC/D RNA结合蛋白的表达，一个标记的神经元，已经离开有丝分裂周期，在细胞迁移从嗅神经上皮细胞向端脑在鸡胚进行了研究，通过免疫荧光和共聚焦激光显微镜。结果显示，该迁移细胞群体是早期的，并且从第一嗅窝阶段开始被a-HuC/D抗体大量标记。在这个发育阶段，嗅觉迁移细胞似乎是唯一的神经元群体，在整个胚胎中表达的HuC/D抗原。在随后的发育阶段中，绝大多数迁移细胞（其数量逐渐增加）继续对α-HuC/D抗体呈高度免疫阳性，而在神经系统的其他区域开始检测到对该抗体的免疫阳性。HuC/D免疫阳性持续到30 HH期（约6.5天），即本研究中研究的后期发育阶段，此时检测到与GnRH的共定位。阴性的抗增殖细胞核抗原（抗PCNA）免疫染色，S期的标志物，表明迁移的嗅细胞已经离开有丝分裂周期。总之，这些结果表明，我们已经确定了第一个人口的有丝分裂后神经元的发育中的神经系统的鸡胚。(C)2003年IBRO。由爱思唯尔有限公司出版。保留所有权利。
In the present study, the expression of the HuC/D RNA-binding proteins, a marker of neurons that have left the mitotic cycle, in cells migrating from the olfactory neuroepithelium toward the telencephalon in the chick embryo was investigated by means of immunofluorescence and confocal laser microscopy. Results showed that this migratory cell population is early and massively labeled by the a-HuC/D antibody starting from the first olfactory pit stage. At this developmental stage, olfactory migratory cells appeared to be the only neuronal population that expressed the HuC/D antigens in the whole embryo. In following developmental stages, the great majority of migratory cells, the number of which increased progressively, continued to be heavily immunopositive for the a-HuC/D antibody while immunopositivity to this antibody begins to be detected in other regions of the nervous system. HuC/D immunopositivity persisted until stage 30 HH (about 6.5 days), the later developmental stage investigated in this study, when colocalization with GnRH was detected. Negativity to the anti-proliferating cell nuclear antigen (anti-PCNA) immunostaining, a marker of S-phase, showed that migratory olfactory cells have left the mitotic cycle. Altogether, these results suggest that we have identified the first population of post-mitotic neurons in the developing nervous system of the chick embryo. (C) 2003 IBRO. Published by Elsevier Ltd. All rights reserved.