Non-degradative dissolution and acetylation of ball-milled plant cell walls: high-resolution solution-state NMR
Non-degradative dissolution and acetylation of ball-milled plant cell walls: high-resolution solution-state NMR
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DOI：
10.1046/j.1365-313x.2003.01817.x
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发表时间：
2003-08-01
期刊：
影响因子：
7.2
通讯作者：
Ralph, J
中科院分区：
文献类型：
--
作者：
Lu, FC;Ralph, J
描述了用于在室温下完全溶解和任选地衍生精细研磨的植物细胞壁材料的两种溶剂系统：二甲基亚砜（DMSO）和四丁基氟化铵（TBAF）或N-甲基咪唑（NMI）。原位乙酰化产生完全可溶于氯仿的乙酰化细胞壁（Ac-CW）。测试的木质素结构保持完全完整。由二维（2D）核磁共振（NMR）实验提供的C-13-H-1相关性的分散揭示了主要的木质素单元，允许整个木质素馏分进行分析的高分辨率溶液状态NMR方法的第一次。非降解细胞壁溶解提供了分析多糖组分的可能性，并通过使用标准均相溶液状态化学来改进当前的细胞壁分析方法。
Two solvent systems for fully dissolving, and optionally derivatizing, finely ground plant cell wall material at room temperature are described: dimethylsulfoxide (DMSO) and tetrabutylammonium fluoride (TBAF) or N-methylimidazole (NMI). In situ acetylation produces acetylated cell walls (Ac-CWs) that are fully soluble in chloroform. Lignin structures tested remain fully intact. The dispersion of C-13-H-1 correlations afforded by two-dimensional (2D) nuclear magnetic resonance (NMR) experiments reveals the major lignin units, allowing the whole lignin fraction to be analyzed by high-resolution solution-state NMR methods for the first time. Non-degradative cell wall dissolution offers the potential to analyze polysaccharide components, and improve current cell wall analytical methods by using standard homogeneous solution-state chemistry.