Carbon nanotubes-based chemiresistive biosensors for detection of microorganisms
Carbon nanotubes-based chemiresistive biosensors for detection of microorganisms
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DOI：
10.1016/j.bios.2010.07.077
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发表时间：
2010-12-15
影响因子：
12.6
通讯作者：
Mulchandani, Ashok
中科院分区：
文献类型：
--
作者：
Garcia-Aljaro, Cristina;Cella, Lakshmi N.;Mulchandani, Ashok
本文报道了基于碳纳米管（CNT）的免疫传感器，用于检测两种类型的微生物，细菌和病毒。以大肠杆菌O157：H7和噬菌体T7分别作为细菌和病毒的模型，以大肠杆菌O157：H7作为病毒的模型。coliK12和噬菌体MS2用于评估生物传感器的选择性。传感元件由平行排列的单壁碳纳米管桥接两个金电极组成，用作化学电阻生物传感器。单壁碳纳米管（SWNTs）功能化的特异性抗体（Ab）的不同微生物通过共价固定到非共价结合的1-芘丁酸琥珀酰亚胺酯。当生物传感器暴露于E. coli O157：H7全细胞或裂解物的检测限分别为10（5）和10（3）CFU（菌落形成单位）/mL，对应于10（3）和10（1）CFU/芯片，而当生物传感器暴露于E. coli K12。在病毒检测的情况下，由于噬菌体与Ab的相互作用，检测到显著的抗性增加，检测限为10（3）PFU/mL，对应于10（1）PFU/芯片，并且对MS2噬菌体具有优异的选择性。该传感器在噬菌体检测中表现出类似于5分钟的快速响应时间，而细菌检测的响应时间为60分钟。（C）2010 Elsevier B.V.保留所有权利。
The paper reports carbon nanotube (CNT)-based immunosensors for the detection of two types of microorganisms, bacteria and viruses. The pathogen Escherichia coli O157:H7 and the bacteriophage T7 were selected as model for bacteria and viruses, respectively, while E. coli K12 and the bacteriophage MS2 were used to assess the selectivity of the biosensor. The transduction element consisted of single-walled carbon nanotubes aligned in parallel bridging two gold electrodes to function as a chemiresistive biosensor. Single-walled carbon nanotubes (SWNTs) were functionalized with specific antibodies (Ab) for the different microorganisms by covalent immobilization to the non-covalently bound 1-pyrene butanoic acid succinimidyl ester. A significant increase in the resistance of the device was observed when the biosensor was exposed to E. coli O157:H7 whole cells or lysates with a limit of detection of 10(5) and 10(3) CFU (colony forming units)/mL, corresponding to 10(3) and 10(1) CFU/chip, respectively, while no response was observed when the biosensor was exposed to the E. coli K12. In the case of virus detection, a significant resistance increase was detected due to interaction of the bacteriophages with the Abs, with a limit of detection of 10(3) PFU/mL corresponding to 10(1) PFU/chip and excellent selectivity against MS2 bacteriophage. The sensor exhibited a fast response time of similar to 5 min in the case of bacteriophage detection, while the response time for the detection of bacteria was 60 min. (C) 2010 Elsevier B.V. All rights reserved.