Chromodomain protein CDYL is required for transmission/restoration of repressive histone marks
Chromodomain protein CDYL is required for transmission/restoration of repressive histone marks
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染色质结构域蛋白 CDYL 是抑制性组蛋白标记的传递/恢复所必需的
DOI：
10.1093/jmcb/mjx013
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发表时间：
2017-06-01
影响因子：
5.5
通讯作者：
Liang, Jing
中科院分区：
文献类型：
--
作者：
Liu, Yongqing;Liu, Shumeng;Liang, Jing
表观遗传信息（例如抑制性组蛋白修饰）的忠实传递或恢复对于维持细胞身份至关重要。我们在此报告，染色质结构域 Y 样蛋白 (CDYL) 是一种含有染色质结构域的转录辅阻遏物，与染色质组装因子 1 (CAF-1) 和复制解旋酶 MCM 复合物物理相关。我们发现 CDYL 桥接 CAF-1 和 MCM，促进 DNA 复制过程中的组蛋白转移和沉积。我们证明 CDYL 将组蛋白修饰酶 G9a、SETDB1 和 EZH2 募集到复制叉，从而在新沉积的组蛋白 H3 上添加 H3K9me2/3 和 H3K27me2/3。值得注意的是，CDYL 的消耗会阻碍早期 S 期进展并使细胞对 DNA 损伤敏感。我们的数据表明 CDYL 在抑制性组蛋白标记的传递/恢复中发挥重要作用，从而保留表观遗传景观以维持细胞身份。
Faithful transmission or restoration of epigenetic information such as repressive histone modifications through generations is critical for the maintenance of cell identity. We report here that chromodomain Y-like protein (CDYL), a chromodomain-containing transcription corepressor, is physically associated with chromatin assembly factor 1 (CAF-1) and the replicative helicase MCM complex. We showed that CDYL bridges CAF-1 and MCM, facilitating histone transfer and deposition during DNA replication. We demonstrated that CDYL recruits histone-modifying enzymes G9a, SETDB1, and EZH2 to replication forks, leading to the addition of H3K9me2/3 and H3K27me2/3 on newly deposited histone H3. Significantly, depletion of CDYL impedes early S phase progression and sensitizes cells to DNA damage. Our data indicate that CDYL plays an important role in the transmission/restoration of repressive histone marks, thereby preserving the epigenetic landscape for the maintenance of cell identity.