Restriction-map variation in the alcohol dehydrogenase region of Drosophila pseudoobscura.
Restriction-map variation in the alcohol dehydrogenase region of Drosophila pseudoobscura.
复制标题
假暗果蝇乙醇脱氢酶区域的限制性图谱变异。
DOI：
10.1093/oxfordjournals.molbev.a040438
复制
发表时间：
1987
影响因子：
10.7
通讯作者：
Anderson,WW
中科院分区：
文献类型：
--
作者：
Schaeffer,SW;Aquadro,CF;Anderson,WW
在 20 个拟暗果蝇品系、1 个果蝇品系和 1 个果蝇品系中，用 6 种限制性内切酶分析了包含 Adh 结构基因的 32 kb 区域。据估计，来自单个群体的 19 个伪暗色螟品系每 15 个核苷酸就有一个具有多态性（p = 0.066）。随机选择的任何两条同源染色体在 48 个（H = 0.021）个核苷酸中就有 1 个是杂合的。在 Adh 转录本上游约 7 kb 处发现了两个 50 和 200 bp 的小插入。高单倍型多样性和低连锁不平衡表明，在该 D.pseudoobscura 种群的历史中，Adh 周围的多态性限制位点几乎是随机分离的，并且近亲繁殖和种群规模周期性减少的影响可以忽略不计。对拟暗果蝇 Adh 区域的限制性内切核酸酶分析与黑腹果蝇中同一区域的强连锁不平衡、高水平的插入/缺失多态性以及较低的核苷酸多态性估计值形成鲜明对比。 Adh 单倍型的系统发育与 D. miranda 的早期分化一致，而 D. persimilis 则属于 D. pseudoobscura 单倍型簇。
A 32-kb region including the Adh structural gene was analyzed with six restriction endonucleases in 20 lines of Drosophila pseudoobscura, one line of D. persimilis, and one line of D. miranda. Nineteen lines of D. pseudoobscura from a single population were estimated to be polymorphic at one in every 15 nucleotides (p = 0.066). Any two homologous chromosomes chosen at random were heterozygous at one in 48 (H = 0.021) nucleotides. Two small insertions of 50 and 200 bp were found approximately 7 kb upstream from the Adh transcript. High haplotype diversity and low linkage disequilibrium suggest that the polymorphic restriction sites around Adh have segregated almost randomly during the history of this D. pseudoobscura population and that the effects of inbreeding and periodic reduction of population size have been negligible. The restriction-endonuclease analysis for the Adh region of D. pseudoobscura stands in sharp contrast to the strong linkage disequilibrium, high levels of insertion/deletion polymorphism, and lower estimates of nucleotide polymorphism found for this same region in D. melanogaster. A phylogeny for the Adh haplotypes is consistent with an early divergence of D. miranda, while D. persimilis falls within the cluster of D. pseudoobscura haplotypes.