Propolis oil extract: quality analysis and evaluation of its antimicrobial activity
Propolis oil extract: quality analysis and evaluation of its antimicrobial activity
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DOI：
10.1080/14786419.2010.529440
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发表时间：
2011-01-01
影响因子：
2.2
通讯作者：
Inkeniene, A. M.
中科院分区：
文献类型：
--
作者：
Ramanauskiene, K.;Inkeniene, A. M.
设计外用蜂胶产品需要确定蜂胶用于皮肤病药物和化妆品制剂的最佳形式。作为一种有效的成分，对从立陶宛采集的原材料中提取的蜂胶油进行了分析。本文首次研究了蜂胶油提取物的流变特性、酚类化合物含量、主要化合物以及抗菌活性。蜂胶油提取物是使用不同溶剂通过浸渍法制备的，原材料采集于立陶宛。含96%乙醇的溶剂混合物提高了流变稳定性和酚类化合物的提取量。高效液相色谱法确定了立陶宛蜂胶的有效质量标志物，即苯丙烷类香草醛、香豆酸和阿魏酸。通过体外实验研究评估了蜂胶油提取物的抗菌活性，并确定了抑制相应微生物的酚类化合物的最低浓度。结果表明，酚类化合物在蜂胶油提取物中具有有效的抗菌活性，因此它可与半固体制剂相容。
Designing propolis products for external use involves determining the optimal form of propolis for the introduction into dermatological pharmaceuticals and cosmetic preparations. As a potent ingredient, propolis oil extract from raw material harvested in Lithuania was analysed. The rheological characteristics, content of phenolic compounds, major compounds and antimicrobial activity of the propolis oil extract are investigated here for the first time. The propolis oil extract was produced by maceration using different solvents, raw material was collected in Lithuania. Solvent mixture with 96% ethanol increased the rheological stability and extracted amount of phenolic compound. High-performance liquid chromatography identified the potent quality markers for Lithuanian propolis, phenylpropanoid vanillin, coumaric acid and ferulic acid. Antimicrobial activity of propolis oil extract was evaluated in experimental studies in vitro, and the minimal concentration of phenolic compounds that inhibited respective microorganisms was determined. The results demonstrate that phenolic compounds have effective antimicrobial activity in propolis oil extract; thus, it can be compatible with the semisolid preparation.