Specific proliferative and antibody responses of premature infants to intestinal colonization with nonpathogenic probiotic E-coli strain Nissle 1917
Specific proliferative and antibody responses of premature infants to intestinal colonization with nonpathogenic probiotic E-coli strain Nissle 1917
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DOI：
10.1046/j.1365-3083.2002.01005.x
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发表时间：
2002-02-01
影响因子：
3.7
通讯作者：
Tlaskalová-Hogenová, H
中科院分区：
文献类型：
--
作者：
Cukrowska, B;Lodínova-Zádníková, R;Tlaskalová-Hogenová, H
本研究的目的是分析口服E. coli Nissle 1917对早产儿全身体液和细胞免疫的影响。34例早产儿感染大肠杆菌。大肠杆菌Nissle 1917在一项随机、安慰剂对照的盲法临床试验中。重复分析婴儿粪便样本中是否存在给药菌株。E.在34名定植婴儿和27名非定植对照的全血中测量大肠杆菌来源。E.大肠杆菌定殖诱导与大肠杆菌的细菌组分一起培养的血细胞的增殖显著增加。coli Nissle 1917和另一株E.大肠杆菌菌株在定植婴儿与非定植对照相比。特异性抗E抗体显著升高。免疫球蛋白（IG）A同种型和非特异性多克隆IgM的大肠杆菌Nissle 1917抗体（Ab）在定殖婴儿的血液中被发现，与非定殖安慰剂对照相比。因此，E. coli Nissle 1917在出生后显著刺激特异性体液和细胞应答，同时诱导非特异性天然免疫。
The aim of this study was to analyze the influence of oral administration of E. coli Nissle 1917 on the systemic humoral and cellular immunity in premature infants. Thirty-four premature infants were colonized with E. coli Nissle 1917 in a randomized, placebo-controlled blinded clinical trial. Stool samples of infants were analyzed repeatedly for the presence of the administered strain. The proliferative response to bacterial antigens of E. coli origin was measured in whole blood of 34 colonized infants and 27 noncolonized controls. E. coli colonization induced a significant increase in the proliferation of blood cells cultivated with bacterial components of E. coli Nissle 1917 and another E. coli strain in colonized infants as compared with noncolonized controls. Significantly higher amounts of specific anti-E. coli Nissle 1917 antibodies (Ab) of immunoglobulin (Ig)A isotype and nonspecific polyclonal IgM were found in the blood of colonized infants compared to noncolonized placebo controls. We concluded that the oral application of E. coli Nissle 1917 after birth significantly stimulates specific humoral and cellular responses and simultaneously induces nonspecific natural immunity.