Cell-free protein synthesis enables one-pot cascade biotransformation in an aqueous-organic biphasic system
Cell-free protein synthesis enables one-pot cascade biotransformation in an aqueous-organic biphasic system
复制标题
无细胞蛋白质合成可在水-有机双相系统中实现一锅级联生物转化
DOI：
10.1002/bit.27541
复制
发表时间：
2020-08-29
影响因子：
3.8
通讯作者：
Li, Jian
中科院分区：
文献类型：
--
作者：
Liu, Wan-Qiu;Wu, Changzhu;Li, Jian
Biocatalytic cascade reactions have become increasingly important and useful for chemical synthesis. However, biocatalysts are often incompatible with organic solvents, which prohibits many cascade reactions involving nonpolar substrates. In this work, we used cell-free protein synthesis (CFPS) to express enzymes in an aqueous-organic biphasic system for the construction of an artificial enzymatic pathway. CFPS-expressed enzymes without purification performed efficiently to convert styrene (below 20 mM) to (S)-1-phenyl-1,2-ethanediol (two steps in one pot) with 100% conversion. In addition, our CFPS system showed great tolerance to different organic solvents and, importantly, the entire biocatalytic system can be consistently scaled up without reduction of the substrate conversion rate. We therefore anticipate that our cell-free approach will make possible cost-effective, high-yielding synthesis of valuable chemicals.
