Expression of MUC5AC and MUC5B mucins in normal and cystic fibrosis lung
Expression of MUC5AC and MUC5B mucins in normal and cystic fibrosis lung
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DOI：
10.1053/rmed.2001.1221
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发表时间：
2002-02-01
影响因子：
4.3
通讯作者：
Chung, KF
中科院分区：
文献类型：
--
作者：
Groneberg, DA;Eynott, PR;Chung, KF
气道粘液分泌过多是慢性气道疾病如囊性纤维化（CF）的特征性特征，并通过损害粘膜纤毛清除和细菌重叠感染导致呼吸衰竭。气道中粘液基质形成家族的粘蛋白的主要组分是MUC5AC和MUC5B。为了研究这些糖蛋白在CF中的表达，对从CF患者获得的气管、支气管和外周肺进行免疫组化，并与正常肺组织进行比较。MUC5AC免疫组化显示上皮衬里的杯状细胞中的信号。此外，杯状细胞内的腺分泌管显示MUC5AC阳性染色。与正常受试者相比，CF切片的特征在于炎性变化和杯状细胞增生，导致MUC5AC阳性细胞数量增加。MUC5B免疫组化染色显示粘膜下腺体和上皮杯状细胞大量染色。在腺体内，免疫反应性仅限于腺粘液细胞，MUC5AC和MUC5B在CF中以与正常组织相比相同的组织学模式表达，由于杯状细胞过度和化生，MUC5AC阳性细胞增加。(C)2001 Elsevier Science Ltd.
Hypersecretion of airway mucus is a characteristic feature of chronic airway diseases like cystic fibrosis (CF) and leads via impairment of the muco-ciliary clearance and bacterial superinfection to respiratory failure. The major components of the mucus matrix forming family of mucins in the airways are MUC5AC and MUC5B. To investigate the expression of these glycoproteins in CF, immunohistochemistry was carried out on trachea, bronchi and peripheral lung obtained from CF patients and compared to normal lung tissues. MUC5AC immunohistochemistry demonstrated signals in goblet cells of the epithelial lining. Also, goblet cells inside glandular secretory ducts revealed MUC5AC-positive staining. In comparison to those from normal subjects, CF sections were characterized by inflammatory changes and goblet cell hyperplasia, resulting in increased numbers of MUC5AC-positive cells. Immunohistochemical staining for MUC5B showed abundant staining of submucosal glands and epithelial goblet cells. Inside the glands, the immunoreactivity was restricted to glandular mucous cells, MUC5AC and MUC5B are expressed in the same histological pattern in CF compared to normal tissues with an increase of MUC5AC-positive cells due to goblet cell hyper- and metaplasia. (C) 2001 Elsevier Science Ltd.