Low-energy laser irradiation affects satellite cell proliferation and differentiation in vitro
Low-energy laser irradiation affects satellite cell proliferation and differentiation in vitro
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DOI：
10.1016/s0167-4889(98)00147-5
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发表时间：
1999-01-11
影响因子：
5.1
通讯作者：
Halevy, O
中科院分区：
文献类型：
--
作者：
Ben-Dov, N;Shefer, G;Halevy, O
低能激光（I-Ie-Ne）照射被发现可以促进体内骨骼肌再生。本研究评估了其对体外卫星细胞增殖和分化的影响。原代大鼠卫星细胞在制备后立即照射不同时间段，并在培养2天后测定胸苷掺入。激光照射以钟形方式影响胸苷掺入，在照射 3 s 时达到峰值。三秒的照射引起细胞周期调节蛋白的诱导：小鼠卫星细胞系中的细胞周期蛋白 D1、细胞周期蛋白 E 和细胞周期蛋白 A、pmi28 以及原代大鼠卫星细胞中的增殖细胞核抗原 (PCNA)。通过激光照射诱导细胞周期蛋白与通过细胞血清再喂养诱导细胞周期蛋白是相容的。激光照射对细胞增殖的影响取决于大鼠的年龄。 3周龄时，受辐射细胞中胸苷的掺入量比对照组高出两倍多，而在6周龄时，这种差异几乎消失。受辐射细胞中的肌球蛋白重链（MHC）蛋白水平比对照细胞低两倍，而受辐射细胞的增殖则高出两倍。与未照射的细胞相比，经过照射的细胞的融合百分比较低。根据这些数据，激光照射对体内骨骼肌再生的促进作用可能是由于其对卫星细胞中早期细胞周期调节基因的激活的影响，导致增殖增加和细胞分化延迟。 (C) 1999 Elsevier Science B.V. 保留所有权利。
Low-energy laser (I-Ie-Ne) irradiation was found to promote skeletal muscle regeneration in vivo. In this study, its effect on the proliferation and differentiation of satellite cells in vitro was evaluated. Primary rat satellite cells were irradiated for various time periods immediately after preparation, and thymidine incorporation was determined after 2 days in culture. Laser irradiation affected thymidine incorporation in a bell-shaped manner, with a peak at 3 s of irradiation. Three seconds of irradiation caused an induction of cell-cycle regulatory proteins: cyclin D1, cyclin E and cyclin A in an established line of mouse satellite cells, pmi28, and proliferating cell nuclear antigen (PCNA) in primary rat satellite cells. The induction of cyclins by laser irradiation was compatible with their induction by serum refeeding of the cells. Laser irradiation effect on cell proliferation was dependent on the rat's age. Al 3 weeks of age, thymidine incorporation in the irradiated cells was more than twofold higher than that in the controls, while at 6 weeks of age this difference had almost disappeared. Myosin heavy chain (MHC) protein levels were twofold lower in the irradiated than in the control cells, whereas the proliferation of the irradiated cells was twofold higher. Fusion percentage was lower in the irradiated compared to non-irradiated cells. In Light of these data, the promoting effect of laser irradiation on skeletal muscle regeneration in vivo may be due to its effect on the activation of early cell-cycle regulatory genes in satellite cells, leading to increased proliferation and to a delay in cell differentiation. (C) 1999 Elsevier Science B.V. All rights reserved.