Hollow Microneedle Arrays for Intradermal Drug Delivery and DNA Electroporation
Hollow Microneedle Arrays for Intradermal Drug Delivery and DNA Electroporation
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DOI：
10.1007/s00232-010-9283-0
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发表时间：
2010-07-01
影响因子：
2.4
通讯作者：
Preat, Veronique
中科院分区：
文献类型：
--
作者：
Daugimont, Lievin;Baron, Nolwenn;Preat, Veronique
微针与引起电穿孔的电脉冲的关联可以导致将药物和DNA有效且更少痛苦地递送到皮肤中。中空导电微针用于（1）无针皮内注射和（2）电脉冲施加，以便在皮肤的表层中实现足以进行电穿孔的电场。将微针阵列与振动插入器组合使用以破坏角质层，从而刺穿皮肤。实现了将蛋白质有效注射到皮肤中，导致针对模型抗原卵清蛋白的免疫应答。然而，当既用作注射微针又用作施加电脉冲的电极时，该装置显示出DNA电转移的几个限制。这可能是由于如数值计算所示的皮肤中的电场分布和/或注射的DNA的低剂量。需要进一步研究这些参数，以优化皮肤中的微创DNA电转移。
The association of microneedles with electric pulses causing electroporation could result in an efficient and less painful delivery of drugs and DNA into the skin. Hollow conductive microneedles were used for (1) needle-free intradermal injection and (2) electric pulse application in order to achieve electric field in the superficial layers of the skin sufficient for electroporation. Microneedle array was used in combination with a vibratory inserter to disrupt the stratum corneum, thus piercing the skin. Effective injection of proteins into the skin was achieved, resulting in an immune response directed to the model antigen ovalbumin. However, when used both as microneedles to inject and as electrodes to apply the electric pulses, the setup showed several limitations for DNA electrotransfer. This could be due to the distribution of the electric field in the skin as shown by numerical calculations and/or the low dose of DNA injected. Further investigation of these parameters is needed in order to optimize minimally invasive DNA electrotransfer in the skin.