Heart regeneration in zebrafish
Heart regeneration in zebrafish
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DOI：
10.1126/science.1077857
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发表时间：
2002-12-13
期刊：
影响因子：
56.9
通讯作者：
Keating, MT
中科院分区：
文献类型：
--
作者：
Poss, KD;Wilson, LG;Keating, MT
哺乳动物和两栖动物的心脏损伤通常会导致疤痕，心肌再生很少。在这里，我们从组织学上证明了斑马鱼在20%心室切除后的2个月内完全再生心脏。再生通过位于新心肌的心外膜前缘的心肌细胞的强烈增殖而发生。斑马鱼的心脏在Mps1有丝分裂检查点激酶（一种关键的细胞周期调节因子）中发生突变，无法再生并形成疤痕。因此，损伤诱导的斑马鱼心肌细胞增殖可以克服瘢痕形成，使心肌再生。这些发现表明，斑马鱼将是有用的基因解剖心脏再生的分子机制。
Cardiac injury in mammals and amphibians typically leads to scarring, with minimal regeneration of heart muscle. Here, we demonstrate histologically that zebrafish fully regenerate hearts within 2 months of 20% ventricular resection. Regeneration occurs through robust proliferation of cardiomyocytes localized at the leading epicardial edge of the new myocardium. The hearts of zebrafish with mutations in the Mps1 mitotic checkpoint kinase, a critical cell cycle regulator, failed to regenerate and formed scars. Thus, injury-induced cardiomyocyte proliferation in zebrafish can overcome scar formation, allowing cardiac muscle regeneration. These findings indicate that zebrafish will be useful for genetically dissecting the molecular mechanisms of cardiac regeneration.