Analysis of structural components and molecular construction of soybean soluble polysaccharides by stepwise enzymatic degradation
Analysis of structural components and molecular construction of soybean soluble polysaccharides by stepwise enzymatic degradation
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DOI：
10.1271/bbb.65.2249
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发表时间：
2001-10-01
影响因子：
1.6
通讯作者：
Yoshimoto, A
中科院分区：
文献类型：
--
作者：
Nakamura, A;Furuta, H;Yoshimoto, A
从大豆子叶中提取的大豆可溶性多糖（SSPS）具有果胶样结构。用四种半纤维素酶和果胶酶处理后的核心多糖含有大致相等数量的L-鼠李糖和D-半乳糖醛酸残基，表明存在由二糖基重复单元-4)-α-D-Ga1pA-(1 -->2)-α-L-Rhap-(1-组成的鼠李糖半乳糖醛酸(RG) I结构。计算出RG链的长度约为15， 28 个和 100 个二糖基重复序列通过半乳糖醛酸 (GN) 链的干预相互连接，构成 SSPS 总糖的 21%，发现所有阿拉伯糖残基都位于 RG 区域的侧链中，占阿拉伯糖残基总量 50% 的半乳糖残基也是如此。半乳糖残基以 α -1,3- 或 α -1,5-阿拉伯聚糖的形式存在，89% 的半乳糖残基以 β -1,4-半乳聚糖的形式存在。
Soybean soluble polysaccharides (SSPS) extracted from soybean cotyledons have a pectin-like structure. The core polysaccharides after treatments with four kinds of hemicellulases and a pectinase contained approximately equal numbers Of L-rhamnose and D-galacturonate residues, suggesting the presence of the rhamnogalacturonan (RG) I structure consisting of the diglycosyl repeating unit, -4)-alpha -D-Ga1pA-(1 -->2)-alpha -L-Rhap-(1-. The lengths of RG chains were calculated as approximately 15, 28, and 100 diglycosyl repeats. The RG components linked to each other by intervention of galacturonan (GN) chains, constituting the backbone of SSPS. All arabinose residues, which constitute 21% of total SSPS sugars, were found to be in side chains from RG regions, and this was also true for galactose residues, which constitute 50% of total sugars. Of arabinose residues, 94% are present as alpha -1,3- or alpha -1,5-arabinans, and 89% of galactose residues were present as beta -1,4-galactans. Galactan chains are modified with arabinose, xylose, fucose, and glucose at the sites close to the RG regions.