Enhancement of biomolecule transport by electroporation:: A review of theory and practical application to transformation of Corynebacterium glutamicum
Enhancement of biomolecule transport by electroporation:: A review of theory and practical application to transformation of Corynebacterium glutamicum
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DOI：
10.1002/bit.20725
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发表时间：
2006-02-20
影响因子：
3.8
通讯作者：
Bustard, MT
中科院分区：
文献类型：
--
作者：
Tryfona, T;Bustard, MT
细胞壁渗透性屏障的选择性和可逆性渗透是许多生物技术应用的焦点。本文从生物、化学和物理三种方法综述了可逆膜渗透的基本原理。重点是电穿孔（电渗透），这往往是最流行的膜渗透和引入外来分子进入细胞的方法。讨论了该方法在工业过程中的应用，以及影响该方法成功的关键因素和参数。本文还总结了多年来为提高工业上重要的氨基酸过量生产细菌谷氨酸棒状杆菌的转化效率而开发的不同策略。(c) 2005 Wiley期刊有限公司
Selective and reversible permeabilization of the cell wall permeability barrier is the focus for many biotechnological applications. In this article, the basic principles for reversible membrane permeabilization, based on biological, chemical, and physical methods are reviewed. Emphasis is given to electroporation (electropermeabilization) which tends to be the most popular method for membrane permeabilization and for introduction of foreign molecules into the cells. The applications of this method in industrial processes as well as the critical factors and parameters which affect the success of this approach are discussed. The different strategies developed throughout the years for increased transformation efficiencies of the industrially important amino acid-overproducing bacterium Corynebacterium glutamicum, are also summarized. (c) 2005 Wiley Periodicals, Inc.