Solid lipid templating of macroporous tissue engineering scaffolds
Solid lipid templating of macroporous tissue engineering scaffolds
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DOI：
10.1016/j.biomaterials.2007.04.018
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发表时间：
2007-08-01
期刊：
影响因子：
14
通讯作者：
Schulz, Michaela B.
中科院分区：
文献类型：
--
作者：
Hacker, Michael;Ringhofer, Michael;Schulz, Michaela B.
大孔可生物降解细胞载体（支架）为体外组织形成提供了三维基质。在这项研究中，我们介绍了利用最近发展的固体脂质模板技术，用不同的可生物降解聚合物制造具有高孔间连通性的大孔支架。从聚合物溶液和固体脂质微粒开始，制备分散体并随后转移到模具中，最后将模具浸入温己烷中沉淀聚合物并提取多孔素。研究显示了如何利用该技术控制支架的孔隙结构、孔径和孔隙率。考虑了分散粘度、孔隙大小和聚合物类型等工艺参数。通过对不同黏度制备的支架进行宏观和微观评价，考察其黏度极限。提出了用振荡流变法对这些数据进行合理化处理的方法。孔隙大小可以通过孔隙颗粒大小和聚合物溶液粘度的适应来控制。孔隙度可以通过改变孔隙素与聚合物的比例来改变。利用已建立的软骨细胞培养模型证明了所得支架的适用性。(c) 2007 Elsevier Ltd.版权所有。
Macroporous biodegradable cell carriers (scaffolds) provide the three-dimensional matrix for tissue formation in vitro. In this study, we present the fabrication of macroporous scaffolds with high inter-pore connectivity from different biodegradable polymers using the recently developed solid lipid templating technique. Starting from a polymer solution and solid lipid microparticles, a dispersion is prepared and subsequently transferred into molds, which are finally Submerged in warm hexane to precipitate the polymer and extract the porogens. The study shows how to control pore structure, pore size and porosity of the scaffold using this technique. The process parameters dispersion viscosity, porogen size and type of polymer are considered. Limits of viscosity are examined by macroscopic and microstructure evaluation of the scaffolds prepared Lit different viscosities. An approach to rationalize these data by oscillation rheometry is shown. Pore size can be controlled by porogen particle size and adaptation of the viscosity of the polymer solution. Porosity can be modified by changing the ratio of porogen to polymer. The Suitability of the resulting scaffolds was shown using an established cartilage cell culture model. (c) 2007 Elsevier Ltd. All rights reserved.